ArticleEpigenetics2016
Differentially methylated obligatory epialleles modulate context-dependent LAM gene expression in the honeybee Apis mellifera.
Article in Epigenetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Genome-wide and gene-specific DNA methylation across developmental stages in Pogonomyrmex californicus: A socially polymorphic ant.Insect molecular biology · 2026Article
- Still no evidence for an environmentally responsive epigenetic clock in an insect.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Comprehensive Genomic Analysis Reveals Novel Transposable Element-Derived MicroRNA Regulating Caste Differentiation in Honeybees.Molecular biology and evolution · 2025Article
- Climate change-induced stress in the honey beeFrontiers in physiology · 2025Review
- DNA Methylation in Honey Bees and the Unresolved Questions in Insect Methylomics.Advances in experimental medicine and biology · 2022Article
- DNA Methylation in Honey Bees and the Unresolved Questions in Insect Methylomics.Advances in experimental medicine and biology · 2022Article
- Screening bioactive food compounds in honey bees suggests curcumin blocks alcohol-induced damage to longevity and DNA methylation.Scientific reports · 2021Article
- Epigenetic Molecular Mechanisms in Insects.Neotropical entomology · 2020Review
- Bumblebee Workers Show Differences in Allele-Specific DNA Methylation and Allele-Specific Expression.Genome biology and evolution · 2020Article
- Exploring DNA Methylation Diversity in the Honey Bee Brain by Ultra-Deep Amplicon Sequencing.Epigenomes · 2020Article
- Unique DNA Methylation Profiles Are Associated with cis-Variation in Honey Bees.Genome biology and evolution · 2019Article
- lncRNA profile of Apis mellifera and its possible role in behavioural transition from nurses to foragers.BMC genomics · 2019Article
- Characterization of a Dopamine Transporter and Its Splice Variant Reveals Novel Features of Dopaminergic Regulation in the Honey Bee.Frontiers in physiology · 2019Article
- Phenotypically distinct female castes in honey bees are defined by alternative chromatin states during larval development.Genome research · 2018Article
- Key Transport and Ammonia Recycling Genes Involved in Aphid Symbiosis Respond to Host-Plant Specialization.G3 (Bethesda, Md.) · 2018Article
- Do social insects support Haig's kin theory for the evolution of genomic imprinting?Epigenetics · 2017Review
- Detecting rare asymmetrically methylated cytosines and decoding methylation patterns in the honeybee genome.Royal Society open science · 2017Article
- Contrasting Sex-and Caste-Dependent piRNA Profiles in the Transposon Depleted Haplodiploid Honeybee Apis mellifera.Genome biology and evolution · 2017Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differential intragenic methylation in social insects has been hailed as a prime mover of environmentally driven organismal plasticity and even as evidence for genomic imprinting. However, very little experimental work has been done to test these ideas and to prove the validity of such claims. Here we analyze in detail differentially methylated obligatory epialleles of a conserved gene encoding lysosomal α-mannosidase (AmLAM) in the honeybee. We combined genotyping of progenies derived from colonies founded by single drone inseminated queens, ultra-deep allele-specific bisulfite DNA sequencing, and gene expression to reveal how sequence variants, DNA methylation, and transcription interrelate. We show that both methylated and non-methylated states of AmLAM follow Mendelian inheritance patterns and are strongly influenced by polymorphic changes in DNA. Increased methylation of a given allele correlates with higher levels of context-dependent AmLAM expression and appears to affect the transcription of an antisense long noncoding RNA. No evidence of allelic imbalance or imprinting involved in this process has been found. Our data suggest that by generating alternate methylation states that affect gene expression, sequence variants provide organisms with a high level of epigenetic flexibility that can be used to select appropriate responses in various contexts. This study represents the first effort to integrate DNA sequence variants, gene expression, and methylation in a social insect to advance our understanding of their relationships in the context of causality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.