ArticleCell cycle (Georgetown, Tex.)2015
Early-late genes of the ecdysone cascade as models for transcriptional studies.
Article in Cell cycle (Georgetown, Tex.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- SRP/dGATAb modulates the ecdysone response bidirectionally via locus-specific regulatory landscape.Epigenetics & chromatin · 2025Article
- Transcriptional induction by ecdysone in Drosophila salivary glands involves an increase in chromatin accessibility and acetylation.Nucleic acids research · 2025Article
- Genetic characterization of candidate ecdysteroid kinases in Drosophila melanogaster.G3 (Bethesda, Md.) · 2024Article
- Coregulators Reside withinInternational journal of molecular sciences · 2023Article
- The negative elongation factor NELF promotes induced transcriptional response of Drosophila ecdysone-dependent genes.Scientific reports · 2021Article
- Ecdysone-Induced 3D Chromatin Reorganization Involves Active Enhancers Bound by Pipsqueak and Polycomb.Cell reports · 2019Article
- Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila.Cell cycle (Georgetown, Tex.) · 2018Article
- Nuclear receptors EcR, Usp, E75, DHR3, and ERR regulate transcription of ecdysone cascade genes.Doklady. Biochemistry and biophysics · 2017Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The DHR3 and Hr4 early-late genes of the ecdysone cascade are described as models for transcriptional studies in Drosophila cells. In a set of experiments, it became clear that these genes are a convenient and versatile system for research into the physiological conditions upon 20-hydroxyecdysone induction. DHR3 and Hr4 gene transcription is characterized by fast activation kinetics, which enables transcriptional studies without the influence of indirect effects. A limited number of activated genes (only 73 genes are induced one hour after treatment) promote the selectivity of transcriptional studies via 20-hydroxyecdysone induction. DHR3 and Hr4 gene expression is dose dependent, is completely controlled by the hormone titer and decreases within hours of 20-hydroxyecdysone withdrawal. The DHR3 and Hr4 gene promoters become functional within 20 minutes after induction, which makes them useful tools for investigation if the early activation process. Their transcription is controlled by the RNA polymerase II pausing mechanism, which is widespread in the genome of Drosophila melanogaster but is still underinvestigated. Uniform expression activation of the DHR3 and Hr4 genes in a cell population was confirmed at both the RNA and protein levels. Homogeneity of the transcription response makes DHR3/Hr4 system valuable for investigation of the protein dynamics during transcription induction.
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