Evidence map›Paper›PMID 26506007›Full record

ArticlePloS one2015

DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil.

Israel de Souza Pinto, Bruna Dias das Chagas, Andressa Alencastre Fuzari Rodrigues, Adelson Luiz Ferreira, Helder Ricas Rezende, Rafaela Vieira Bruno, Aloisio Falqueto, José Dilermando Andrade-Filho, Eunice Aparecida Bianchi Galati, Paloma Helena Fernandes Shimabukuro and 2 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
6.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 67 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Israel de Souza PintoLab. de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Brasil Ave. 4365, 21040360, Rio de Janeiro, RJ, Brazil; Unidade de Medicina Tropical, UFES, Marechal Campos Ave. 1468, 29043900, Vitória, Espírito Santo, Brazil.
Bruna Dias das ChagasLab. de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Brasil Ave. 4365, 21040360, Rio de Janeiro, RJ, Brazil.
Andressa Alencastre Fuzari RodriguesLab. de Doenças Parasitárias, Instituto Oswaldo Cruz, FIOCRUZ, Brasil Ave. 4365, 21040360, Rio de Janeiro, RJ, Brazil.
Adelson Luiz FerreiraUnidade de Medicina Tropical, UFES, Marechal Campos Ave. 1468, 29043900, Vitória, Espírito Santo, Brazil.
Helder Ricas RezendeUnidade de Medicina Tropical, UFES, Marechal Campos Ave. 1468, 29043900, Vitória, Espírito Santo, Brazil.
Rafaela Vieira BrunoLab. de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Brasil Ave. 4365, 21040360, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular - INCT-EM/CNPq, Rio de Janeiro, Brazil.
Aloisio FalquetoUnidade de Medicina Tropical, UFES, Marechal Campos Ave. 1468, 29043900, Vitória, Espírito Santo, Brazil.
José Dilermando Andrade-FilhoGrupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, FIOCRUZ, Augusto de Lima Ave. 1715, 30190002, Belo Horizonte, MG, Brazil.
Eunice Aparecida Bianchi GalatiDepartamento de Epidemiologia, Faculdade de Saúde Pública, USP, Dr. Arnaldo Cerqueira Cesar Ave. 715, 01246904, São Paulo, SP, Brazil.
Paloma Helena Fernandes ShimabukuroGrupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, FIOCRUZ, Augusto de Lima Ave. 1715, 30190002, Belo Horizonte, MG, Brazil.
Reginaldo Peçanha BrazilLab. de Doenças Parasitárias, Instituto Oswaldo Cruz, FIOCRUZ, Brasil Ave. 4365, 21040360, Rio de Janeiro, RJ, Brazil.
Alexandre Afranio PeixotoLab. de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Brasil Ave. 4365, 21040360, Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA barcoding has been an effective tool for species identification in several animal groups. Here, we used DNA barcoding to discriminate between 47 morphologically distinct species of Brazilian sand flies. DNA barcodes correctly identified approximately 90% of the sampled taxa (42 morphologically distinct species) using clustering based on neighbor-joining distance, of which four species showed comparatively higher maximum values of divergence (range 4.23-19.04%), indicating cryptic diversity. The DNA barcodes also corroborated the resurrection of two species within the shannoni complex and provided an efficient tool to differentiate between morphologically indistinguishable females of closely related species. Taken together, our results validate the effectiveness of DNA barcoding for species identification and the discovery of cryptic diversity in sand flies from Brazil.

Indexed as

DNA Barcoding, TaxonomicAnimalsBrazilDNAFemalePsychodidaeSpecies SpecificityDNA

Identifiers

PMID26506007
PMCPMC4624639
OpenAlexW2097722906

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.