ArticleJournal of mass spectrometry : JMS2015
Decellularization of intact tissue enables MALDI imaging mass spectrometry analysis of the extracellular matrix.
Article in Journal of mass spectrometry : JMS, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 41 citations in OpenAlex.
- Enhancing the Sensitivity of Mass Spectrometry Imaging through Spatial Signal Averaging.Journal of the American Society for Mass Spectrometry · 2026Article
- Rapid and efficient in situ digestion of proteins by SoniCoat for MALDI mass spectrometry imaging.Analytical and bioanalytical chemistry · 2026Article
- MALDI mass spectrometry imaging of extracellular matrix proteins.The Analyst · 2025Review
- Cell-Instructive Biomaterials with Native-Like Biochemical Complexity.Annual review of biomedical engineering · 2025Review
- Optimization of Collagenase Proteomics for Improved Mass Spectrometry Imaging Peptide Identification.Analytical chemistry · 2025Article
- Decellularization and Enzymatic Digestion Methods to Enhance ECM Protein Detection via MALDI-MS Imaging.Analytical chemistry · 2025Article
- Article
- A Proof of Principle 2D Spatial Proteome Mapping Analysis Reveals Distinct Regional Differences in the Cardiac Proteome.Life (Basel, Switzerland) · 2024Article
- Review
- Evaluation of antibody-based single cell type imaging techniques coupled to multiplexed imaging of N-glycans and collagen peptides by matrix-assisted laser desorption/ionization mass spectrometry imaging.Analytical and bioanalytical chemistry · 2023Article
- Unraveling the Relevance of Tissue-Specific Decellularized Extracellular Matrix Hydrogels for Vocal Fold Regenerative Biomaterials: A Comprehensive Proteomic and In Vitro Study.Advanced nanobiomed research · 2023Article
- Basal lamina changes in neurodegenerative disorders.Molecular neurodegeneration · 2021Review
- Analysis of pancreatic extracellular matrix protein post-translational modificationsMolecular omics · 2021Article
- The Extracellular Matrix in Pancreatic Cancer: Description of a Complex Network and Promising Therapeutic Options.Cancers · 2021Review
- Building Organs Using Tissue-Specific Microenvironments: Perspectives from a Bioprosthetic Ovary.Trends in biotechnology · 2021Review
- 3D Mapping Reveals a Complex and Transient Interstitial Matrix During Murine Kidney Development.Journal of the American Society of Nephrology : JASN · 2021Article
- Multiplexed imaging mass spectrometry of the extracellular matrix using serial enzyme digests from formalin-fixed paraffin-embedded tissue sections.Analytical and bioanalytical chemistry · 2021Article
- The matrix in cancer.Nature reviews. Cancer · 2021Review
- Time-of-flight secondary ion mass spectrometry three-dimensional imaging of surface modifications in poly(caprolactone) scaffold pores.Journal of biomedical materials research. Part A · 2019Article
- A recommended and verified procedure for in situ tryptic digestion of formalin-fixed paraffin-embedded tissues for analysis by matrix-assisted laser desorption/ionization imaging mass spectrometry.Journal of mass spectrometry : JMS · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful molecular mapping technology that offers unbiased visualization of the spatial arrangement of biomolecules in tissue. Although there has been a significant increase in the number of applications employing this technology, the extracellular matrix (ECM) has received little attention, likely because ECM proteins are mostly large, insoluble and heavily cross-linked. We have developed a new sample preparation approach to enable MALDI IMS analysis of ECM proteins in tissue. Prior to freezing and sectioning, intact tissues are decellularized by incubation in sodium dodecyl sulfate. Decellularization removes the highly abundant, soluble species that dominate a MALDI IMS spectrum while preserving the structural integrity of the ECM. In situ tryptic hydrolysis and imaging of tryptic peptides are then carried out to accommodate the large sizes of ECM proteins. This new approach allows the use of MALDI IMS for identification of spatially specific changes in ECM protein expression and modification in tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.