Evidence map›Paper›PMID 26505218›Full record

ArticleMolecular endocrinology (Baltimore, Md.)2015

Novel CARM1-Interacting Protein, DZIP3, Is a Transcriptional Coactivator of Estrogen Receptor-α.

Daniel J Purcell, Swati Chauhan, Diane Jimenez-Stinson, Kathleen R Elliott, Tenzin D Tsewang, Young-Ho Lee, Brian Marples, David Y Lee

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular endocrinology (Baltimore, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Daniel J PurcellSection of Radiation Oncology (D.J.P., S.C., D.J.-S., K.R.E., T.D.T., D.Y.L.), Division of Hematology-Oncology, Department of Internal Medicine, and University of New Mexico Comprehensive Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131; Department of Biochemistry (Y.-H.L.), Keck School of Medicine, University of Southern California. Los Angeles, California 90089; and Department of Radiation Oncology (B.M.), William Beaumont Hospital, Royal Oak, Michigan 48073.
Swati ChauhanSection of Radiation Oncology (D.J.P., S.C., D.J.-S., K.R.E., T.D.T., D.Y.L.), Division of Hematology-Oncology, Department of Internal Medicine, and University of New Mexico Comprehensive Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131; Department of Biochemistry (Y.-H.L.), Keck School of Medicine, University of Southern California. Los Angeles, California 90089; and Department of Radiation Oncology (B.M.), William Beaumont Hospital, Royal Oak, Michigan 48073.
Diane Jimenez-StinsonSection of Radiation Oncology (D.J.P., S.C., D.J.-S., K.R.E., T.D.T., D.Y.L.), Division of Hematology-Oncology, Department of Internal Medicine, and University of New Mexico Comprehensive Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131; Department of Biochemistry (Y.-H.L.), Keck School of Medicine, University of Southern California. Los Angeles, California 90089; and Department of Radiation Oncology (B.M.), William Beaumont Hospital, Royal Oak, Michigan 48073.
Kathleen R ElliottSection of Radiation Oncology (D.J.P., S.C., D.J.-S., K.R.E., T.D.T., D.Y.L.), Division of Hematology-Oncology, Department of Internal Medicine, and University of New Mexico Comprehensive Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131; Department of Biochemistry (Y.-H.L.), Keck School of Medicine, University of Southern California. Los Angeles, California 90089; and Department of Radiation Oncology (B.M.), William Beaumont Hospital, Royal Oak, Michigan 48073.
Tenzin D TsewangSection of Radiation Oncology (D.J.P., S.C., D.J.-S., K.R.E., T.D.T., D.Y.L.), Division of Hematology-Oncology, Department of Internal Medicine, and University of New Mexico Comprehensive Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131; Department of Biochemistry (Y.-H.L.), Keck School of Medicine, University of Southern California. Los Angeles, California 90089; and Department of Radiation Oncology (B.M.), William Beaumont Hospital, Royal Oak, Michigan 48073.
Young-Ho LeeSection of Radiation Oncology (D.J.P., S.C., D.J.-S., K.R.E., T.D.T., D.Y.L.), Division of Hematology-Oncology, Department of Internal Medicine, and University of New Mexico Comprehensive Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131; Department of Biochemistry (Y.-H.L.), Keck School of Medicine, University of Southern California. Los Angeles, California 90089; and Department of Radiation Oncology (B.M.), William Beaumont Hospital, Royal Oak, Michigan 48073.
Brian MarplesSection of Radiation Oncology (D.J.P., S.C., D.J.-S., K.R.E., T.D.T., D.Y.L.), Division of Hematology-Oncology, Department of Internal Medicine, and University of New Mexico Comprehensive Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131; Department of Biochemistry (Y.-H.L.), Keck School of Medicine, University of Southern California. Los Angeles, California 90089; and Department of Radiation Oncology (B.M.), William Beaumont Hospital, Royal Oak, Michigan 48073.
David Y LeeSection of Radiation Oncology (D.J.P., S.C., D.J.-S., K.R.E., T.D.T., D.Y.L.), Division of Hematology-Oncology, Department of Internal Medicine, and University of New Mexico Comprehensive Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131; Department of Biochemistry (Y.-H.L.), Keck School of Medicine, University of Southern California. Los Angeles, California 90089; and Department of Radiation Oncology (B.M.), William Beaumont Hospital, Royal Oak, Michigan 48073.
New Mexico Cancer Center · USBeaumont Hospital, Royal Oak · USUniversity of New Mexico · USUniversity of Southern California · USUNM Comprehensive Cancer Center

Funding

WOMEN'S CANCERS RESEARCH PROGRAMP30CA118100 · NCI · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Yolanda Sanchez · 2005 to 2026
$57.1M
NCI NIH HHS 5P30CA118100NCI NIH HHS P30 CA118100
6 · The paper itself

Abstract

Coactivator-associated arginine methyltransferase 1 (CARM1) is known to promote estrogen receptor (ER)α-mediated transcription in breast cancer cells. To further characterize the regulation of ERα-mediated transcription by CARM1, we screened CARM1-interacting proteins by yeast two-hybrid. Here, we have identified an E3 ubiquitin ligase, DAZ (deleted in azoospermia)-interacting protein 3 (DZIP3), as a novel CARM1-binding protein. DZIP3-dependent ubiquitination of histone H2A has been associated with repression of transcription. However, ERα reporter gene assays demonstrated that DZIP3 enhanced ERα-mediated transcription and cooperated synergistically with CARM1. Interaction with CARM1 was observed with the E3 ligase RING domain of DZIP3. The methyltransferase activity of CARM1 partially contributed to the synergy with DZIP3 for transcription activation, but the E3 ubiquitin ligase activity of DZIP3 was dispensable. DZIP3 also interacted with the C-terminal activation domain 2 of glucocorticoid receptor-interacting protein 1 (GRIP1) and enhanced the interaction between GRIP1 and CARM1. Depletion of DZIP3 by small interfering RNA in MCF7 cells reduced estradiol-induced gene expression of ERα target genes, GREB1 and pS2, and DZIP3 was recruited to the estrogen response elements of the same ERα target genes. These results indicate that DZIP3 is a novel coactivator of ERα target gene expression.

Indexed as

AnimalsCarrier ProteinsCell LineCOS CellsEstrogen Receptor alphaHumansMiceMice, KnockoutNerve Tissue ProteinsProtein-Arginine N-MethyltransferasesProtein BindingRNA-Binding ProteinsTranscriptional ActivationUbiquitin-Protein LigasesCarrier Proteinscoactivator-associated arginine methyltransferase 1DZIP3 protein, humanESR1 protein, humanEstrogen Receptor alphaGRIP1 protein, humanNerve Tissue ProteinsProtein-Arginine N-MethyltransferasesRNA-Binding ProteinsUbiquitin-Protein Ligases

Identifiers

PMID26505218
PMCPMC4664229
OpenAlexW1825058767

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.