ArticleACS nano2015
Single-Cell Quantification of Cytosine Modifications by Hyperspectral Dark-Field Imaging.
Article in ACS nano, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 58 citations in OpenAlex.
- New approach methodologies for next-generation risk assessment of nanomaterials and nano-enabled products.Nano convergence · 2026Review
- In vitro hyperspectral biomarkers of human chondrosarcoma cells in nanoparticle-mediated radiosensitization using carbon ions.Scientific reports · 2023Article
- Optical Methods for Label-Free Detection of Bacteria.Biosensors · 2022Review
- Flexible hyperspectral surface plasmon resonance microscopy.Nature communications · 2022Article
- Multivariate Imaging for Fast Evaluation of In Situ Dark Field Microscopy Hyperspectral Data.Molecules (Basel, Switzerland) · 2022Article
- Safety Evaluation of Nanotechnology Products.Pharmaceutics · 2021Review
- Article
- Multi and transgenerational epigenetic effects of di-(2-ethylhexyl) phthalate (DEHP) in liver.Toxicology and applied pharmacology · 2020Article
- Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology.Micromachines · 2020Article
- Optical Interrogation Techniques for Nanophotonic Biochemical Sensors.Sensors (Basel, Switzerland) · 2019Review
- Ultrasound beam steering of oxygen nanobubbles for enhanced bladder cancer therapy.Scientific reports · 2018Article
- Single-Cell Analysis Using Hyperspectral Imaging Modalities.Journal of biomechanical engineering · 2018Review
- Technical advances in global DNA methylation analysis in human cancers.Journal of biological engineering · 2017Review
- Optical Clearing Delivers Ultrasensitive Hyperspectral Dark-Field Imaging for Single-Cell Evaluation.ACS nano · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Epigenetic modifications on DNA, especially on cytosine, play a critical role in regulating gene expression and genome stability. It is known that the levels of different cytosine derivatives are highly dynamic and are regulated by a variety of factors that act on the chromatin. Here we report an optical methodology based on hyperspectral dark-field imaging (HSDFI) using plasmonic nanoprobes to quantify the recently identified cytosine modifications on DNA in single cells. Gold (Au) and silver (Ag) nanoparticles (NPs) functionalized with specific antibodies were used as contrast-generating agents due to their strong local surface plasmon resonance (LSPR) properties. With this powerful platform we have revealed the spatial distribution and quantity of 5-carboxylcytosine (5caC) at the different stages in cell cycle and demonstrated that 5caC was a stably inherited epigenetic mark. We have also shown that the regional density of 5caC on a single chromosome can be mapped due to the spectral sensitivity of the nanoprobes in relation to the interparticle distance. Notably, HSDFI enables an efficient removal of the scattering noises from nonspecifically aggregated nanoprobes, to improve accuracy in the quantification of different cytosine modifications in single cells. Further, by separating the LSPR fingerprints of AuNPs and AgNPs, multiplex detection of two cytosine modifications was also performed. Our results demonstrate HSDFI as a versatile platform for spatial and spectroscopic characterization of plasmonic nanoprobe-labeled nuclear targets at the single-cell level for quantitative epigenetic screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.