ArticleBiomedical optics express2015
Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration.
Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Characterization of inner ear granular bodies by polarization-resolved second harmonic generation microscopy.Biomedical optics express · 2026Article
- Polarimetric second harmonic generation microscopy of partially oriented fibers II: Imaging study.Biophysical journal · 2023Article
- Polarimetric second-harmonic generation microscopy of partially oriented fibers I: Digital modeling.Biophysical journal · 2023Article
- Multiphoton microscopy is a nondestructive label-free approach to investigate the 3D structure of gas cell walls in bread dough.Scientific reports · 2023Article
- Investigation into the structure of crystalline maltodextrin particles by second harmonic generation microscopy.Biomedical optics express · 2023Article
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- Nondestructive circadian profiling of starch content in fresh intact Arabidopsis leaf with two-photon fluorescence and second-harmonic generation imaging.Scientific reports · 2022Article
- Second harmonic generation microscopy of otoconia.Biomedical optics express · 2022Article
- Spectroscopic second and third harmonic generation microscopy using a femtosecond laser source in the third near-infrared (NIR-III) optical window.Biomedical optics express · 2022Article
- Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy.Sensors (Basel, Switzerland) · 2019Article
- Second Harmonic Generation of Unpolarized Light.Physical review letters · 2017Article
- Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy.Scientific reports · 2017Article
- Connection of Jones and Mueller Tensors in Second Harmonic Generation and Multi-Photon Fluorescence Measurements.The journal of physical chemistry. B · 2016Article
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Authors and funding
8 authors.
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Abstract
Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R'SHG ) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R'SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.
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