ArticleHorticulture research2014
Transcriptome of Erysiphe necator-infected Vitis pseudoreticulata leaves provides insight into grapevine resistance to powdery mildew.
Article in Horticulture research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Genomic and Transcriptomic Analyses Provide Insights Into Erysiphe necator Pathogenicity and Grapevine Response.Plant biotechnology journal · 2026Article
- Article
- Emerging Roles of Epigenetics in Grapevine and Winegrowing.Plants (Basel, Switzerland) · 2024Review
- A newly identified glycosyltransferase AsRCOM provides resistance to purple curl leaf disease in agave.BMC genomics · 2023Article
- Powdery Mildew Resistance Genes in Vines: An Opportunity to Achieve a More Sustainable Viticulture.Pathogens (Basel, Switzerland) · 2022Review
- PRGdb 4.0: an updated database dedicated to genes involved in plant disease resistance process.Nucleic acids research · 2022Article
- The grape powdery mildew resistance lociFrontiers in plant science · 2022Article
- Transcriptomic and metabolomic integration as a resource in grapevine to study fruit metabolite quality traits.Frontiers in plant science · 2022Article
- Characterization of physiological and antioxidant responses inFrontiers in plant science · 2022Article
- Genetic changes in the genusFrontiers in plant science · 2022Review
- Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries.Plant methods · 2021Article
- Identification and Characterization of Downy Mildew-Responsive microRNAs in IndianJournal of fungi (Basel, Switzerland) · 2021Article
- Investigation of long non-coding RNAs as regulatory players of grapevine response to powdery and downy mildew infection.BMC plant biology · 2021Article
- Transcriptome Analysis and Cell Morphology ofGenes · 2021Article
- Arbuscular Mycorrhizal Symbiosis Triggers Major Changes in Primary Metabolism Together With Modification of Defense Responses and Signaling in Both Roots and Leaves ofFrontiers in plant science · 2021Article
- The Molecular Priming of Defense Responses is Differently Regulated in Grapevine Genotypes Following Elicitor Application against Powdery Mildew.International journal of molecular sciences · 2020Article
- Recent advances in biotechnological studies on wild grapevines as valuable resistance sources for smart viticulture.Molecular biology reports · 2020Review
- Grape-RNA: A Database for the Collection, Evaluation, Treatment, and Data Sharing of Grape RNA-Seq Datasets.Genes · 2020Article
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Authors and funding
6 authors.
Funding
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Abstract
Powdery mildew (PM), which is caused by the pathogen Erysiphe necator (Schw.) Burr., is the single most damaging disease of cultivated grapes (Vitis vinifera) worldwide. However, little is known about the transcriptional response of grapes to infection with PM. RNA-seq analysis was used for deep sequencing of the leaf transcriptome to study PM resistance in Chinese wild grapes (V. pseudoreticulata Baihe 35-1) to better understand the interaction between host and pathogen. Greater than 100 million (M) 90-nt cDNA reads were sequenced from a cDNA library derived from PM-infected leaves. Among the sequences obtained, 6541 genes were differentially expressed (DEG) and were annotated with Gene Ontology terms and by pathway enrichment. The significant categories that were identified included the following: defense, salicylic acid (SA) and jasmonic acid (JA) responses; systemic acquired resistance (SAR); hypersensitive response; plant-pathogen interaction; flavonoid biosynthesis; and plant hormone signal transduction. Various putative secretory proteins were identified, indicating potential defense responses to PM infection. In all, 318 putative R-genes and 183 putative secreted proteins were identified, including the defense-related R-genes BAK1, MRH1 and MLO3 and the defense-related secreted proteins GLP and PR5. The expression patterns of 16 genes were further illuminated by RT-qPCR. The present study identified several candidate genes and pathways that may contribute to PM resistance in grapes and illustrated that RNA-seq is a powerful tool for studying gene expression. The RT-qPCR results reveal that effective resistance responses of grapes to PM include enhancement of JA and SAR responses and accumulation of phytoalexins.
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Registered trials
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