ArticleProceedings of the National Academy of Sciences of the United States of America2015
Transcriptional read-through is not sufficient to induce an epigenetic switch in the silencing activity of Polycomb response elements.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Multi-gene Co-expression systems inSynthetic and systems biotechnology · 2026Review
- Vostok: A looping factor for the organization of the regulatory genome in the Drosophila brain.Molecular cell · 2025Article
- Nucleoporin Elys attaches peripheral chromatin to the nuclear pores in interphase nuclei.Communications biology · 2024Article
- Polycomb protein binding and looping in the ON transcriptional state.Science advances · 2024Article
- Context-dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila.Genetics · 2023Article
- Crol contributes to PRE-mediated repression and Polycomb group proteins recruitment in Drosophila.Nucleic acids research · 2023Article
- Recruitment to Chromatin of (GA)n-Associated Factors GAF and Psq in the Transgenic Model System Depends on the Presence of Architectural Protein Binding Sites.Doklady. Biochemistry and biophysics · 2022Article
- Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins.Cellular and molecular life sciences : CMLS · 2022Article
- A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals.Nature genetics · 2021Article
- Boundaries potentiate polycomb response element-mediated silencing.BMC biology · 2021Article
- RNA-DNA strand exchange by the Drosophila Polycomb complex PRC2.Nature communications · 2020Article
- A human somatic cell culture system for modelling gene silencing by transcriptional interference.Heliyon · 2020Article
- A Novel PRE-Element from Drosophila virilis Genome as a Useful Model Silencer.Doklady. Biochemistry and biophysics · 2019Article
- Sex combs reduced (Scr) regulatory region of Drosophila revisited.Molecular genetics and genomics : MGG · 2017Article
- Polycomb and Trithorax Group Genes inGenetics · 2017Review
- Single vector non-leaky gene expression system for Drosophila melanogaster.Scientific reports · 2017Article
- Slow Chromatin Dynamics Allow Polycomb Target Genes to Filter Fluctuations in Transcription Factor Activity.Cell systems · 2017Article
- The bxdPRE/TRE element terminates passing through transcription.Doklady. Biochemistry and biophysics · 2017Article
- PRE/TRE elements act as transcription activators in Drosophila S2 Cells.Doklady. Biochemistry and biophysics · 2017Article
- The GAGA factor regulatory network: Identification of GAGA factor associated proteins.PloS one · 2017Article
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Abstract
In Drosophila, Polycomb (PcG) and Trithorax (TrxG) group proteins are assembled on Polycomb response elements (PREs) to maintain tissue and stage-specific patterns of gene expression. Critical to coordinating gene expression with the process of differentiation, the activity of PREs can be switched "on" and "off." When on, the PRE imposes a silenced state on the genes in the same domain that is stably inherited through multiple rounds of cell division. When the PRE is switched off, the domain is in a state permissive for gene expression that can be stably inherited. Previous studies have suggested that a burst of transcription through a PRE sequence displaces PcG proteins and provides a universal mechanism for inducing a heritable switch in PRE activity from on to off; however, the evidence favoring this model is indirect. Here, we have directly tested the transcriptional read-through mechanism. Contrary to previous suggestions, we show that transcription through the PRE is not sufficient for inducing an epigenetic switch in PRE activity. In fact, even high levels of continuous transcription through a PRE fails to dislodge the PcG proteins, nor does it remove repressive histone marks. Our results indicate that other mechanisms involving adjacent DNA regulatory elements must be implicated in heritable switch of PRE activity.
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