ArticleProceedings of the National Academy of Sciences of the United States of America2015
Effects of pressure on the dynamics of an oligomeric protein from deep-sea hyperthermophile.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Unravelling the Adaptation Mechanisms to High Pressure in Proteins.International journal of molecular sciences · 2022Article
- Mesophilic Pyrophosphatase Function at High Temperature: A Molecular Dynamics Simulation Study.Biophysical journal · 2020Article
- The effects of pressure on the energy landscape of proteins.Scientific reports · 2018Article
- The Photosystem II Subunit S under Stress.Biophysical journal · 2017Article
- Quasi-elastic Neutron Scattering Reveals Ligand-Induced Protein Dynamics of a G-Protein-Coupled Receptor.The journal of physical chemistry letters · 2016Article
- Accelerating Enzymatic Catalysis Using Vortex Fluidics.Angewandte Chemie (International ed. in English) · 2016Article
- High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes.Scientific reports · 2016Article
- China's first pulsed neutron source.Nature materials · 2016Article
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6 authors.
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Abstract
Inorganic pyrophosphatase (IPPase) from Thermococcus thioreducens is a large oligomeric protein derived from a hyperthermophilic microorganism that is found near hydrothermal vents deep under the sea, where the pressure is up to 100 MPa (1 kbar). It has attracted great interest in biophysical research because of its high activity under extreme conditions in the seabed. In this study, we use the quasielastic neutron scattering (QENS) technique to investigate the effects of pressure on the conformational flexibility and relaxation dynamics of IPPase over a wide temperature range. The β-relaxation dynamics of proteins was studied in the time ranges from 2 to 25 ps, and from 100 ps to 2 ns, using two spectrometers. Our results indicate that, under a pressure of 100 MPa, close to that of the native environment deep under the sea, IPPase displays much faster relaxation dynamics than a mesophilic model protein, hen egg white lysozyme (HEWL), at all measured temperatures, opposite to what we observed previously under ambient pressure. This contradictory observation provides evidence that the protein energy landscape is distorted by high pressure, which is significantly different for hyperthermophilic (IPPase) and mesophilic (HEWL) proteins. We further derive from our observations a schematic denaturation phase diagram together with energy landscapes for the two very different proteins, which can be used as a general picture to understand the dynamical properties of thermophilic proteins under pressure.
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