ArticleProceedings of the National Academy of Sciences of the United States of America2015
Semisynthetic protein nanoreactor for single-molecule chemistry.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Controlled assembly and sophisticated additive manufacturing of macroscopic droplets.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Understanding single-molecule reactions using nanopore-based techniques.Nature chemistry · 2025Review
- Medical and Nonmedical Applications of Synthetic Transmembrane Anion Transporters.Chemical reviews · 2025Review
- Nanopore analysis of salvianolic acids in herbal medicines.Nature communications · 2024Article
- Article
- Assembly of transmembrane pores from mirror-image peptides.Nature communications · 2022Article
- Profiling single-molecule reaction kinetics under nanopore confinement.Chemical science · 2022Article
- Functionalised nanopores: chemical and biological modifications.Chemical science · 2022Review
- Programmable nano-reactors for stochastic sensing.Nature communications · 2021Article
- Rapid and multiplex preparation of engineeredChemical science · 2021Article
- A pH-independent quiet OmpG pore with enhanced electrostatic repulsion among the extracellular loops.Biochimica et biophysica acta. Biomembranes · 2021Article
- Single-Molecule Observation of Intermediates in Bioorthogonal 2-Cyanobenzothiazole Chemistry.Angewandte Chemie (International ed. in English) · 2020Article
- Transmembrane Epitope Delivery by Passive Protein Threading through the Pores of the OmpF Porin Trimer.Journal of the American Chemical Society · 2020Article
- Chemoenzymatic Semisynthesis of Proteins.Chemical reviews · 2020Review
- Single molecule observation of hard-soft-acid-base (HSAB) interaction in engineeredChemical science · 2019Article
- Construction of supramolecular nanotubes from protein crystals.Chemical science · 2019Article
- Manipulating and visualizing the dynamic aggregation-induced emission within a confined quartz nanopore.Nature communications · 2018Article
- Single-Molecule Titration in a Protein Nanoreactor Reveals the Protonation/Deprotonation Mechanism of a C:C Mismatch in DNA.Journal of the American Chemical Society · 2018Article
- Nanopore Analysis of the 5-Guanidinohydantoin to Iminoallantoin Isomerization in Duplex DNA.The Journal of organic chemistry · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The covalent chemistry of individual reactants bound within a protein pore can be monitored by observing the ionic current flow through the pore, which acts as a nanoreactor responding to bond-making and bond-breaking events. In the present work, we incorporated an unnatural amino acid into the α-hemolysin (αHL) pore by using solid-phase peptide synthesis to make the central segment of the polypeptide chain, which forms the transmembrane β-barrel of the assembled heptamer. The full-length αHL monomer was obtained by native chemical ligation of the central synthetic peptide to flanking recombinant polypeptides. αHL pores with one semisynthetic subunit were then used as nanoreactors for single-molecule chemistry. By introducing an amino acid with a terminal alkyne group, we were able to visualize click chemistry at the single-molecule level, which revealed a long-lived (4.5-s) reaction intermediate. Additional side chains might be introduced in a similar fashion, thereby greatly expanding the range of single-molecule covalent chemistry that can be investigated by the nanoreactor approach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.