ArticleJournal of lipid research2015
ApoA-I Milano stimulates lipolysis in adipose cells independently of cAMP/PKA activation.
Article in Journal of lipid research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Emerging Therapeutic Targets for Acute Coronary Syndromes: Novel Advancements and Future Directions.Biomedicines · 2024Review
- Differential renal proteomics analysis in a novel rat model of iodinated contrast-induced acute kidney injury.Renal failure · 2023Article
- Predictive value of ApoB/ApoA-I for recurrence within 1 year after first incident stroke.Frontiers in neurology · 2023Article
- Causal relationship between circulating lipid traits and periodontitis: univariable and multivariable Mendelian randomization.Frontiers in endocrinology · 2023Article
- Adipose cell size changes are associated with a drastic actin remodeling.Scientific reports · 2019Article
- Redox regulation of hormone sensitive lipase: Potential role in the mechanism of MEHP-induced stimulation of basal steroid synthesis in MA-10 Leydig cells.Reproductive toxicology (Elmsford, N.Y.) · 2019Article
- Intact glucose uptake despite deteriorating signaling in adipocytes with high-fat feeding.Journal of molecular endocrinology · 2018Article
- Visualization of lipid directed dynamics of perilipin 1 in human primary adipocytes.Scientific reports · 2017Article
- Rosiglitazone drives cavin-2/SDPR expression in adipocytes in a CEBPα-dependent manner.PloS one · 2017Article
- Exendin-4 Ameliorates Lipotoxicity-induced Glomerular Endothelial Cell Injury by Improving ABC Transporter A1-mediated Cholesterol Efflux in Diabetic apoE Knockout Mice.The Journal of biological chemistry · 2016Article
- The unsolved mystery of apoA-I recycling in adipocyte.Lipids in health and disease · 2016Review
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ApoA-I, the main protein component of HDL, is suggested to be involved in metabolic homeostasis. We examined the effects of Milano, a naturally occurring ApoA-I variant, about which little mechanistic information is available. Remarkably, high-fat-fed mice treated with Milano displayed a rapid weight loss greater than ApoA-I WT treated mice, and a significantly reduced adipose tissue mass, without an inflammatory response. Further, lipolysis in adipose cells isolated from mice treated with either WT or Milano was increased. In primary rat adipose cells, Milano stimulated cholesterol efflux and increased glycerol release, independently of β-adrenergic stimulation and phosphorylation of hormone sensitive lipase (Ser563) and perilipin (Ser522). Stimulation with Milano had a significantly greater effect on glycerol release compared with WT but similar effect on cholesterol efflux. Pharmacological inhibition or siRNA silencing of ABCA1 did not diminish Milano-stimulated lipolysis, although binding to the cell surface was decreased, as analyzed by fluorescence microscopy. Interestingly, methyl-β-cyclodextrin, a well-described cholesterol acceptor, dose-dependently stimulated lipolysis. Together, these results suggest that decreased fat mass and increased lipolysis following Milano treatment in vivo is partly explained by a novel mechanism at the adipose cell level comprising stimulation of lipolysis independently of the canonical cAMP/protein kinase A signaling pathway.
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