Evidence map›Paper›PMID 26503787›Full record

ArticleMolecular and cellular biology2016

High Fractional Occupancy of a Tandem Maf Recognition Element and Its Role in Long-Range β-Globin Gene Regulation.

Jared R Stees, Mir A Hossain, Tomoki Sunose, Yasushi Kudo, Carolina E Pardo, Nancy H Nabilsi, Russell P Darst, Rosha Poudyal, Kazuhiko Igarashi, Suming Huang and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Jared R SteesDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA.
Mir A HossainDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA.
Tomoki SunoseDepartment of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
Yasushi KudoDepartment of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
Carolina E PardoDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA.
Nancy H NabilsiDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA.
Russell P DarstDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA.
Rosha PoudyalDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA.
Kazuhiko IgarashiDepartment of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan CREST, Japan Science and Technology Agency, Sendai, Miyagi, Japan.
Suming HuangDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA sumingh@ufl.edu jbungert@ufl.edu.
Michael P KladdeDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA.
Jörg BungertDepartment of Biochemistry and Molecular Biology, Center for Epigenetics, Genetics Institute, UF Health Cancer Center, Powell-Gene Therapy Center, University of Florida, Gainesville, Florida, USA sumingh@ufl.edu jbungert@ufl.edu.
University of Florida Health · USTohoku University · JPJapan Science and Technology Agency · JP

Funding

STRUCTURE/FUNCTION OF THE HUMAN B-GLOBIN LCRR01DK052356 · NIDDK · UNIVERSITY OF FLORIDA · PI BUNGERT, JORG · 1997 to 2017
$4.7M
Regulation of insulator function and globin gene expression by USF and associatedR01HL091929 · NHLBI · UNIVERSITY OF FLORIDA · PI HUANG, SUMING · 2009 to 2013
$2.0M
Transcriptional regulation of TAL1/SCL in normal and malignant hematopoiesisR01HL090589 · NHLBI · UNIVERSITY OF FLORIDA · PI HUANG, SUMING · 2008 to 2012
$1.9M
Interplay of Epigenetic Mechanisms in Gene SilencingR01CA155390 · NCI · UNIVERSITY OF FLORIDA · PI KLADDE, MICHAEL PAUL · 2011 to 2015
$1.5M
Functional proteomics in differentiating erythroid cellsR01DK083389 · NIDDK · UNIVERSITY OF FLORIDA · PI BUNGERT, JORG, STROUBOULIS, IOANNIS JOHN · 2010 to 2013
$1.1M
NCI NIH HHS R01 CA155390NHLBI NIH HHS R01 HL090589NHLBI NIH HHS R01HL090589NHLBI NIH HHS R01 HL091929NHLBI NIH HHS R01HL091929NIDDK NIH HHS R01 DK052356NIDDK NIH HHS R01DK052356NIDDK NIH HHS R01DK083389
6 · The paper itself

Abstract

Enhancers and promoters assemble protein complexes that ultimately regulate the recruitment and activity of RNA polymerases. Previous work has shown that at least some enhancers form stable protein complexes, leading to the formation of enhanceosomes. We analyzed protein-DNA interactions in the murine β-globin gene locus using the methyltransferase accessibility protocol for individual templates (MAPit). The data show that a tandem Maf recognition element (MARE) in locus control region (LCR) hypersensitive site 2 (HS2) reveals a remarkably high degree of occupancy during differentiation of mouse erythroleukemia cells. Most of the other transcription factor binding sites in LCR HS2 or in the adult β-globin gene promoter regions exhibit low fractional occupancy, suggesting highly dynamic protein-DNA interactions. Targeting of an artificial zinc finger DNA-binding domain (ZF-DBD) to the HS2 tandem MARE caused a reduction in the association of MARE-binding proteins and transcription complexes at LCR HS2 and the adult βmajor-globin gene promoter but did not affect expression of the βminor-globin gene. The data demonstrate that a stable MARE-associated footprint in LCR HS2 is important for the recruitment of transcription complexes to the adult βmajor-globin gene promoter during erythroid cell differentiation.

Indexed as

Gene Expression Regulation, LeukemicLocus Control RegionTranscriptional ActivationAnimalsbeta-GlobinsCell DifferentiationCell Line, TumorDNAErythroid CellsGene Expression RegulationLeukemia, Erythroblastic, AcuteMicePromoter Regions, GeneticRNA Polymerase IITranscription FactorsZinc Fingersbeta-GlobinsDNARNA Polymerase IITranscription Factors

Identifiers

PMID26503787
PMCPMC4719303
OpenAlexW2232244945

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.