ArticleThe Journal of general physiology2015
Altered conductance and permeability of Cx40 mutations associated with atrial fibrillation.
Article in The Journal of general physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Genetically engineered human embryonic kidney cells as a novel vehicle for dual patch clamp study of human gap junction channels.The Biochemical journal · 2024Article
- Therapeutic advances in atrial fibrillation based on animal models.Journal of Zhejiang University. Science. B · 2024Review
- LncRNA GAS5 Attenuates Cardiac Electrical Remodeling Induced by Rapid Pacing via the miR-27a-3p/HOXa10 Pathway.International journal of molecular sciences · 2023Article
- Cytoskeletal Protein Variants Driving Atrial Fibrillation: Potential Mechanisms of Action.Cells · 2022Review
- Pinocembrin Decreases Atrial Fibrillation Susceptibility in a Rodent Model of Depression.Frontiers in cardiovascular medicine · 2022Article
- Mechanisms of Connexin Regulating Peptides.International journal of molecular sciences · 2021Review
- Molecular Insights in Atrial Fibrillation Pathogenesis and Therapeutics: A Narrative Review.Diagnostics (Basel, Switzerland) · 2021Review
- Four-State Model for Simulating Kinetic and Steady-State Voltage-Dependent Gating of Gap Junctions.Biophysical journal · 2020Article
- Overview of the Components of Cardiac Metabolism.Drug metabolism and disposition: the biological fate of chemicals · 2019Review
- Electrical coupling and its channels.The Journal of general physiology · 2018Review
- Connexins and Atrial Fibrillation in Obstructive Sleep Apnea.Current sleep medicine reports · 2018Article
- Specific mutations in the permease domain of septal protein SepJ differentially affect functions related to multicellularity in the filamentous cyanobacteriumMicrobial cell (Graz, Austria) · 2018Article
- Value of multilocus genetic risk score for atrial fibrillation in end-stage kidney disease patients in a Polish population.Scientific reports · 2018Article
- Connexins and Pannexins in Vascular Function and Disease.International journal of molecular sciences · 2018Review
- Functional Characterization of Novel Atrial Fibrillation-Linked GJA5 (Cx40) Mutants.International journal of molecular sciences · 2018Article
- Regulation of cardiac gap junctions by protein phosphatases.Journal of molecular and cellular cardiology · 2017Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Gap junctions ensure the rapid propagation of the action potential throughout the myocardium. Three mutant forms of connexin40 (Cx40; A96S, M163V, and G38D), the primary component of the atrial gap junction channel, are associated with atrial fibrillation and retain the ability to form functional channels. We determined the biophysical properties of these mutant gap junctions in transiently transfected HeLa and N2A cells. All three mutants showed macroscopic junctional conductances over the range of 0.5 to 40 nS, and voltage dependences comparable to those of wild-type (WT) Cx40. However, the unitary conductance of G38D channels was ∼1.6-fold higher than that of WT Cx40 channels (∼220 vs. ∼135 pS), whereas the unitary conductances of the A96S and M163V mutants were similar to that of WT Cx40. Furthermore, the M163V and G38D channels exhibited approximately two- and approximately fivefold higher permeability to the anionic dye Lucifer yellow (LY) relative to K+ (LY/K+) compared with that of WT Cx40, whereas A96S LY transfer was similar to that of WT (G38D > M163V > A96S ≈ Cx40WT). In contrast, G38D channels were almost impermeable to cationic ethidium bromide (EtBr), suggesting that G38D alters channel selectivity. Conversely, A96S and M163V channels showed enhanced EtBr permeability relative to WT Cx40, with the following permeability order: M163V > A96S > Cx40WT > G38D. Altered conductive and permeability properties of mutant channels suggest an essential role for Cx40-mediated biochemical and electrical coupling in cardiac tissues. The altered properties of the three single-base substitution mutants may play a role in mechanisms of reentry arrhythmias.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.