ArticleScientific reports2015
Early aggregation preceding the nucleation of insulin amyloid fibrils as monitored by small angle X-ray scattering.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 73 citations in OpenAlex.
- Optical imaging and spectroscopic characterization of subvisible particles in protein therapeutics.Advanced drug delivery reviews · 2026Review
- Spatially constrained disulfide bond shuffling delays insulin aggregation and enhances neurotoxicity.Nature communications · 2025Article
- Antibody Aggregation: A Problem Within the Biopharmaceutical Industry and Its Role in AL Amyloidosis Disease.The protein journal · 2025Review
- Chemical screening by time-resolved X-ray scattering to discover allosteric probes.Nature chemical biology · 2024Article
- Structural basis of insulin fibrillation.Science advances · 2023Article
- An Investigation into the Acidity-Induced Insulin Agglomeration: Implications for Drug Delivery and Translation.ACS omega · 2023Article
- Carbon dots as a versatile tool to monitor insulin aggregation.Analytical and bioanalytical chemistry · 2023Article
- Amyloid Fibrillation of Insulin: Amelioration Strategies and Implications for Translation.ACS pharmacology & translational science · 2022Review
- Controlling Amyloid Fibril Properties Via Ionic Liquids: The Representative Case of Ethylammonium Nitrate and Tetramethylguanidinium Acetate on the Amyloidogenesis of Lysozyme.The journal of physical chemistry letters · 2022Article
- A New Approach to Supramolecular Structure Determination in Pharmaceutical Preparation of Self-Assembling Peptides: A Case Study of Lanreotide Autogel.Pharmaceutics · 2022Article
- The intriguing dose-dependent effect of selected amphiphilic compounds on insulin amyloid aggregation: Focus on a cholesterol-based detergent, Chobimalt.Frontiers in molecular biosciences · 2022Article
- Trehalose Effect on the Aggregation of Model Proteins into Amyloid Fibrils.Life (Basel, Switzerland) · 2020Article
- Diverse Structural Conversion and Aggregation Pathways of Alzheimer's Amyloid-β (1-40).ACS nano · 2019Article
- Congo Red and amyloids: history and relationship.Bioscience reports · 2019Review
- Recent progress on understanding the mechanisms of amyloid nucleation.Biophysical reviews · 2018Review
- Article
- A specific form of prefibrillar aggregates that functions as a precursor of amyloid nucleation.Scientific reports · 2018Article
- The emergence of superstructural order in insulin amyloid fibrils upon multiple rounds of self-seeding.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleation event of amyloid fibrils is one of the most crucial processes that dictate the timing and rate of the pathology of diseases; however, information regarding how protein molecules associate to produce fibril nuclei is currently limited. In order to explore this issue in more detail, we performed time-resolved small angle X-ray scattering (SAXS) measurements on insulin fibrillation, in combination with additional multidirectional analyses of thioflavin T fluorescence, FTIR spectroscopy, light scattering, and light transmittance, during the fibrillation process of bovine insulin. SAXS monitoring revealed that insulin molecules associated into rod-like prefibrillar aggregates in the very early stage of the reaction. After the formation of these early aggregates, they appeared to further coalesce mutually to form larger clusters, and the SAXS profiles subsequently showed the further time evolution of conformational development towards mature amyloid fibrils. Distinct types of structural units in terms of shape in a nano-scale order, cross-β content, and thioflavin T fluorescence intensity were observed in a manner that was dependent on the fibrillation pathways. These results suggest the presence of diverse substructures that characterize various fibrillation pathways, and eventually, manifest polymorphisms in mature amyloid fibrils.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.