ReviewPlant biotechnology journal2016
Oligonucleotide-directed mutagenesis for precision gene editing.
Review in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 152 citations in OpenAlex.
- Market introduction of plant varieties and products with gene-edited traits.GM crops & food · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Review
- Advancements in Plant-Derived sRNAs Therapeutics: Classification, Delivery Strategies, and Therapeutic Applications.International journal of molecular sciences · 2025Review
- Genome editing in grain legumes for food security.Frontiers in genome editing · 2025Review
- Genome editing in Sub-Saharan Africa: a game-changing strategy for climate change mitigation and sustainable agriculture.GM crops & food · 2024Review
- The Global Advance of Genome-Edited Plants to the Market: The Key Role of Chile in Its Development.Plants (Basel, Switzerland) · 2024Article
- CRISPR-Based Editing of thePlants (Basel, Switzerland) · 2024Article
- Review
- CRISPR/Cas9 Mutagenesis through Introducing a Nanoparticle Complex Made of a Cationic Polymer and Nucleic Acids into Maize Protoplasts.International journal of molecular sciences · 2023Article
- Genome Editing and Improvement of Abiotic Stress Tolerance in Crop Plants.Life (Basel, Switzerland) · 2023Review
- The economics and policy of genome editing in crop improvement.The plant genome · 2023Review
- CRISPR-Cas engineering in food science and sustainable agriculture: recent advancements and applications.Bioprocess and biosystems engineering · 2023Review
- Oligo-Not Only for Silencing: Overlooked Potential for Multidirectional Action in Plants.International journal of molecular sciences · 2023Review
- Engineering plant immune circuit: walking to the bright future with a novel toolbox.Plant biotechnology journal · 2023Review
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops.International journal of molecular sciences · 2022Review
- Advances in S gene targeted genome-editing and its applicability to disease resistance breeding in selectedBioengineered · 2022Article
- Novel Plant Breeding Techniques Shake Hands with Cereals to Increase Production.Plants (Basel, Switzerland) · 2022Review
- Genome Editing: A Promising Approach for Achieving Abiotic Stress Tolerance in Plants.International journal of genomics · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleotide-directed mutagenesis (ODM), one of the many tools of Cibus' Rapid Trait Development System (RTDS(™) ) technology, offers a rapid, precise and non-transgenic breeding alternative for trait improvement in agriculture to address this urgent need. This review explores the application of ODM as a precision genome editing technology, with emphasis on using oligonucleotides to make targeted edits in plasmid, episomal and chromosomal DNA of bacterial, fungal, mammalian and plant systems. The process of employing ODM by way of RTDS technology has been improved in many ways by utilizing a fluorescence conversion system wherein a blue fluorescent protein (BFP) can be changed to a green fluorescent protein (GFP) by editing a single nucleotide of the BFP gene (CAC→TAC; H66 to Y66). For example, dependent on oligonucleotide length, applying oligonucleotide-mediated technology to target the BFP transgene in Arabidopsis thaliana protoplasts resulted in up to 0.05% precisely edited GFP loci. Here, the development of traits in commercially relevant plant varieties to improve crop performance by genome editing technologies such as ODM, and by extension RTDS, is reviewed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.