ArticleStem cell research & therapy2015
Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis.
Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Efficacy and safety of mesenchymal stem cells for cerebral infarction: A meta-analysis.Biomedical reports · 2026Article
- Osteogenic Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells on Composite Polymeric Scaffolds: A Review.Current stem cell research & therapy · 2025Review
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- TMEM175 plays a crucial role in osteoblast differentiation by regulating lysosomal function and autophagy.Molecules and cells · 2024Article
- Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate-A Retrospective Analysis of 58 Patients.Biomedicines · 2023Article
- Systematic review of articular cartilage derived chondroprogenitors for cartilage repair in animal models.Journal of orthopaedics · 2023Review
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- Supplementation of articular cartilage-derived chondroprogenitors with bone morphogenic protein-9 enhances chondrogenesis without affecting hypertrophy.Biotechnology letters · 2022Article
- Characterization of Induction and Targeting of Senescent Mesenchymal Stromal Cells.Tissue engineering. Part C, Methods · 2022Article
- Tissue Engineering with Compact Bone-Derived Cell Spheroids Enables Bone Formation around Transplanted Tooth.Tissue engineering and regenerative medicine · 2022Article
- DNMT1 is a negative regulator of osteogenesis.Biology open · 2022Article
- Effect of TNF-α and IL-6 on Compact Bone-Derived Cells.Tissue engineering and regenerative medicine · 2021Article
- Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering.Tissue engineering. Part C, Methods · 2021Article
- Isolation and characterization of mesenchymal stem cells in orthopaedics and the emergence of compact bone mesenchymal stem cells as a promising surgical adjunct.World journal of stem cells · 2020Review
- Effect of short-term betamethasone administration on the regeneration process of tissue-engineered bone.Histology and histopathology · 2020Article
- Co-culture of the bone and bone marrow: a novel way to obtain mesenchymal stem cells with enhanced osteogenic ability for fracture healing in SD rats.Journal of orthopaedic surgery and research · 2019Article
- Bone Repair and Regenerative Biomaterials: Towards Recapitulating the Microenvironment.Polymers · 2019Review
- Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability.Stem cells international · 2019Article
- Mesenchymal Stem Cells in the Musculoskeletal System: From Animal Models to Human Tissue Regeneration?Stem cell reviews and reports · 2018Review
- Novel therapeutic strategies for degenerative disc disease: Review of cell biology and intervertebral disc cell therapy.SAGE open medicine · 2018Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionMesenchymal stem cells (MSCs) hold great promise for regenerative therapies in the musculoskeletal system. Although MSCs from bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) have been extensively characterized, there is still debate as to the ideal source of MSCs for tissue-engineering applications in bone repair.
methodsMSCs were isolated from cortical bone fragments (CBF-MSCs) obtained from patients undergoing laminectomy, selected by fluorescence-activated cell sorting analysis, and tested for their potential to undergo mesodermic differentiation. CBF-MSCs were then compared with BM-MSCs and AD-MSCs for their colony-forming unit capability and osteogenic potential in both normoxia and hypoxia. After 2 and 4 weeks in inducing media, differentiation was assessed qualitatively and quantitatively by the evaluation of alkaline phosphatase (ALP) expression and mineral deposition (Von Kossa staining). Transcriptional activity of osteoblastogenesis-associated genes (Alp, RUNX2, Spp1, and Bglap) was also analyzed.
resultsThe cortical fraction of the bone contains a subset of cells positive for MSC-associated markers and capable of tri-lineage differentiation. The hypoxic conditions were generally more effective in inducing osteogenesis for the three cell lines. However, at 2 and 4 weeks, greater calcium deposition and ALP expression were observed in both hypoxic and normoxic conditions in CBF-MSCs compared with AD- and BM-MSCs. These functional observations were further corroborated by gene expression analysis, which showed a significant upregulation of Bglap, Alp, and Spp1, with a 22.50 (±4.55)-, 46.56 (±7.4)-, 71.46 (±4.16)-fold increase compared with their uninduced counterparts.
conclusionsThis novel population of MSCs retains a greater biosynthetic activity in vitro, which was found increased in hypoxic conditions. The present study demonstrates that quantitative differences between MSCs retrieved from bone marrow, adipose, and the cortical portion of the bone with respect to their osteogenic potential exist and suggests the cortical bone as suitable candidate to use for orthopedic tissue engineering and regenerative medicine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.