ArticleBMC genomics2015
A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 21 citations in OpenAlex.
- Genomic characterization and drug resistance of Bordetella pseudohinzii first isolated from wild niviventer.BMC microbiology · 2025Article
- Non-classicalThe Journal of international medical research · 2024Article
- Natural History and Ecology of Interactions BetweenFrontiers in cellular and infection microbiology · 2022Article
- Evolution and Conservation ofFrontiers in microbiology · 2020Review
- Genotypic and phenotypic adaptation of pathogens: lesson from the genus Bordetella.Current opinion in infectious diseases · 2019Review
- A model of chronic, transmissible Otitis Media in mice.PLoS pathogens · 2019Article
- Conservation of Ancient Genetic Pathways for Intracellular Persistence Among Animal Pathogenic Bordetellae.Frontiers in microbiology · 2019Article
- Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice.Scientific reports · 2018Article
- Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia.The Journal of veterinary medical science · 2018Article
- Draft genome ofGenomics data · 2017Article
- Evolution of Bordetellae from Environmental Microbes to Human Respiratory Pathogens: Amoebae as a Missing Link.Frontiers in cellular and infection microbiology · 2017Article
- Environmental Origin of the GenusFrontiers in microbiology · 2017Article
- Cross-Foster Rederivation Compared with Antibiotic Administration in the Drinking Water to EradicateJournal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice.International journal of systematic and evolutionary microbiology · 2016Article
- Complete Genome Sequences of Bordetella flabilis, Bordetella bronchialis, and "Bordetella pseudohinzii".Genome announcements · 2016Article
- Acquisition and loss of virulence-associated factors during genome evolution and speciation in three clades of Bordetella species.BMC genomics · 2016Article
- Bordetella pseudohinzii as a Confounding Organism in Murine Models of Pulmonary Disease.Comparative medicine · 2016Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundClustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria. These systems provide adaptive immunity against mobile genetic elements specified by the spacer sequences stored within the CRISPR.
methodsThe CRISPR-Cas system has been identified using Basic Local Alignment Search Tool (BLAST) against other sequenced and annotated genomes and confirmed via CRISPRfinder program. Using Polymerase Chain Reactions (PCR) and Sanger DNA sequencing, we discovered CRISPRs in additional bacterial isolates of the same species of Bordetella. Transcriptional activity and processing of the CRISPR have been assessed via RT-PCR.
resultsHere we describe a novel Type II-C CRISPR and its associated genes-cas1, cas2, and cas9-in several isolates of a newly discovered Bordetella species. The CRISPR-cas locus, which is absent in all other Bordetella species, has a significantly lower GC-content than the genome-wide average, suggesting acquisition of this locus via horizontal gene transfer from a currently unknown source. The CRISPR array is transcribed and processed into mature CRISPR RNAs (crRNA), some of which have homology to prophages found in closely related species B. hinzii.
conclusionsExpression of the CRISPR-Cas system and processing of crRNAs with perfect homology to prophages present in closely related species, but absent in that containing this CRISPR-Cas system, suggest it provides protection against phage predation. The 3,117-bp cas9 endonuclease gene from this novel CRISPR-Cas system is 990 bp smaller than that of Streptococcus pyogenes, the 4,017-bp allele currently used for genome editing, and which may make it a useful tool in various CRISPR-Cas technologies.
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