ArticleEnvironmental health perspectives2016
Parabens and Human Epidermal Growth Factor Receptor Ligand Cross-Talk in Breast Cancer Cells.
Article in Environmental health perspectives, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 69 citations in OpenAlex.
- Parabens and estrogen-mediated cancers: unraveling coincidence, co-occurrence, and causality.npj women's health · 2026Review
- Transgenerational inheritance of diminished ovarian reserve triggered by prenatal propylparaben exposure in mice.Nature communications · 2025Article
- Review
- Parabens Promote Protumorigenic Effects in Luminal Breast Cancer Cell Lines With Diverse Genetic Ancestry.Journal of the Endocrine Society · 2023Article
- 2',3',4'-Trihydroxychalcone changes estrogen receptor α regulation of genes and breast cancer cell proliferation by a reprogramming mechanism.Molecular medicine (Cambridge, Mass.) · 2022Article
- Minireview: Parabens Exposure and Breast Cancer.International journal of environmental research and public health · 2022Review
- Hair product use and breast cancer incidence in the Black Women's Health Study.Carcinogenesis · 2021Article
- The sex-specific association between maternal urinary paraben levels and offspring size at birth.Environmental science and pollution research international · 2021Article
- Urinary concentrations of parabens amongst Iranian adults and their associations with socio-demographic factors.Journal of environmental health science & engineering · 2020Article
- A comprehensive analysis of racial disparities in chemical biomarker concentrations in United States women, 1999-2014.Environment international · 2020Article
- Use of skincare products and risk of cancer of the breast and endometrium: a prospective cohort study.Environmental health : a global access science source · 2019Article
- Environmental exposures, stem cells, and cancer.Pharmacology & therapeutics · 2019Review
- Traces of intact paraben molecules in endometrial carcinoma.Environmental science and pollution research international · 2019Article
- Urinary concentrations of environmental phenols and their associations with breast cancer incidence and mortality following breast cancer.Environment international · 2019Article
- Homology models of mouse and rat estrogen receptor-Computational toxicology (Amsterdam, Netherlands) · 2019Article
- State of the evidence 2017: an update on the connection between breast cancer and the environment.Environmental health : a global access science source · 2017Review
- MYC-Driven Pathways in Breast Cancer Subtypes.Biomolecules · 2017Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundXenoestrogens are synthetic compounds that mimic endogenous estrogens by binding to and activating estrogen receptors. Exposure to estrogens and to some xenoestrogens has been associated with cell proliferation and an increased risk of breast cancer. Despite evidence of estrogenicity, parabens are among the most widely used xenoestrogens in cosmetics and personal-care products and are generally considered safe. However, previous cell-based studies with parabens do not take into account the signaling cross-talk between estrogen receptor α (ERα) and the human epidermal growth factor receptor (HER) family.
objectivesWe investigated the hypothesis that the potency of parabens can be increased with HER ligands, such as heregulin (HRG).
methodsThe effects of HER ligands on paraben activation of c-Myc expression and cell proliferation were determined by real-time polymerase chain reaction, Western blots, flow cytometry, and chromatin immunoprecipitation assays in ERα- and HER2-positive human BT-474 breast cancer cells.
resultsButylparaben (BP) and HRG produced a synergistic increase in c-Myc mRNA and protein levels in BT-474 cells. Estrogen receptor antagonists blocked the synergistic increase in c-Myc protein levels. The combination of BP and HRG also stimulated proliferation of BT-474 cells compared with the effects of BP alone. HRG decreased the dose required for BP-mediated stimulation of c-Myc mRNA expression and cell proliferation. HRG caused the phosphorylation of serine 167 in ERα. BP and HRG produced a synergistic increase in ERα recruitment to the c-Myc gene.
conclusionOur results show that HER ligands enhanced the potency of BP to stimulate oncogene expression and breast cancer cell proliferation in vitro via ERα, suggesting that parabens might be active at exposure levels not previously considered toxicologically relevant from studies testing their effects in isolation. CITATION: Pan S, Yuan C, Tagmount A, Rudel RA, Ackerman JM, Yaswen P, Vulpe CD, Leitman DC. 2016. Parabens and human epidermal growth factor receptor ligand cross-talk in breast cancer cells. Environ Health Perspect 124:563-569; http://dx.doi.org/10.1289/ehp.1409200.
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