ArticleMethods in molecular biology (Clifton, N.J.)2016
Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries.
Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Substrate-Derived Peptides for Selective Covalent Inhibition of Protein Tyrosine Kinases.ACS chemical biology · 2026Article
- Substrate-derived peptides for selective covalent inhibition of protein tyrosine kinases.bioRxiv : the preprint server for biology · 2026Article
- DeepKinome: quantitative prediction of kinase binding affinity by a compound using deep learning based regression model.Frontiers in molecular biosciences · 2025Article
- Effects of heterologous kinase domains on growth factor receptor specificity.Cellular signalling · 2024Article
- PTMoreR-enabled cross-species PTM mapping and comparative phosphoproteomics across mammals.Cell reports methods · 2024Article
- Post-translational modifications in the Protein Data Bank.Acta crystallographica. Section D, Structural biology · 2024Review
- Kinase-Catalyzed Crosslinking and Immunoprecipitation (K-CLIP) to Explore Kinase-Substrate Pairs.Current protocols · 2022Article
- ACONITASE 3 is part of theANAC017 transcription factor-dependent mitochondrial dysfunction response.Plant physiology · 2021Article
- Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function.Cell reports · 2021Article
- Differential effects of putative N-glycosylation sites in human Tau on Alzheimer's disease-related neurodegeneration.Cellular and molecular life sciences : CMLS · 2021Article
- A Phytophthora sojae CRN effector mediates phosphorylation and degradation of plant aquaporin proteins to suppress host immune signaling.PLoS pathogens · 2021Article
- Structure of a GRK5-Calmodulin Complex Reveals Molecular Mechanism of GRK Activation and Substrate Targeting.Molecular cell · 2021Article
- RORα phosphorylation by casein kinase 1α as glucose signal to regulate estrogen sulfation in human liver cells.The Biochemical journal · 2020Article
- CRK2 and C-terminal Phosphorylation of NADPH Oxidase RBOHD Regulate Reactive Oxygen Species Production in Arabidopsis.The Plant cell · 2020Article
- Comprehensive profiling of the STE20 kinase family defines features essential for selective substrate targeting and signaling output.PLoS biology · 2019Article
- Kinase Substrate Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library.Biochemistry · 2018Article
- A high throughput assay to identify substrate-selective inhibitors of the ERK protein kinases.Biochemical pharmacology · 2017Article
- Identification of the phosphorylation targets of symbiotic receptor-like kinases using a high-throughput multiplexed assay for kinase specificity.The Plant journal : for cell and molecular biology · 2017Article
- Multiplex Substrate Profiling by Mass Spectrometry for Kinases as a Method for Revealing Quantitative Substrate Motifs.Analytical chemistry · 2017Article
- Structure of the Human Protein Kinase ZAK in Complex with Vemurafenib.ACS chemical biology · 2016Article
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2 authors.
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Abstract
Eukaryotic protein kinases phosphorylate substrates at serine, threonine, and tyrosine residues that fall within the context of short sequence motifs. Knowing the phosphorylation site motif for a protein kinase facilitates designing substrates for kinase assays and mapping phosphorylation sites in protein substrates. Here, we describe an arrayed peptide library protocol for rapidly determining kinase phosphorylation consensus sequences. This method uses a set of peptide mixtures in which each of the 20 amino acid residues is systematically substituted at nine positions surrounding a central site of phosphorylation. Peptide mixtures are arrayed in multiwell plates and analyzed by radiolabel assay with the kinase of interest. The preferred sequence is determined from the relative rate of phosphorylation of each peptide in the array. Consensus peptides based on these sequences typically serve as efficient and specific kinase substrates for high-throughput screening or incorporation into biosensors.
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