ArticleFrontiers in plant science2015
Internal transcribed spacer 2 barcode: a good tool for identifying Acanthopanacis cortex.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- The rps15-rpl32 intergenic locus is a phylogeographic marker for Latin American Zea mays landrace varieties and subspecies.BMC genomics · 2025Article
- DNA barcoding combined with high-resolution melting analysis to discriminate rhubarb species and its traditional Chinese patent medicines.Frontiers in pharmacology · 2024Article
- Assessing diversity and phylogeny of Indonesian breadfruit (Artocarpus spp.) using internal transcribed spacer (ITS) region and leaf morphology.Journal, genetic engineering & biotechnology · 2023Article
- Article
- Comparative Analysis of Acanthopanacis Cortex and Periplocae Cortex Using an Electronic Nose and Gas Chromatography-Mass Spectrometry Coupled with Multivariate Statistical Analysis.Molecules (Basel, Switzerland) · 2022Article
- DNA barcoding: an efficient technology to authenticate plant species of traditional Chinese medicine and recent advances.Chinese medicine · 2022Review
- Challenges in Medicinal and Aromatic Plants DNA Barcoding-Lessons from the Lamiaceae.Plants (Basel, Switzerland) · 2022Review
- Article
- The screening and identification of DNA barcode sequences for Rehmannia.Scientific reports · 2019Article
- DetectingGenes · 2019Article
- Molecular Authentication of the Medicinal Species ofFrontiers in plant science · 2019Article
- Identifying the Species of Seeds in Traditional Chinese Medicine Using DNA Barcoding.Frontiers in pharmacology · 2018Article
- Establishment of the most comprehensive ITS2 barcode database to date of the traditional medicinal plant Rhodiola (Crassulacaee).Scientific reports · 2017Article
- Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market.Scientific reports · 2017Article
- Advances of Community-Level Plant DNA Barcoding in China.Frontiers in plant science · 2017Article
- Inspecting the True Identity of Herbal Materials fromFrontiers in plant science · 2017Article
- A Nucleotide Signature for the Identification of Angelicae Sinensis Radix (Danggui) and Its Products.Scientific reports · 2016Article
- Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets.Molecules (Basel, Switzerland) · 2016Article
- Application of the Ribosomal DNA ITS2 Region of Physalis (Solanaceae): DNA Barcoding and Phylogenetic Study.Frontiers in plant science · 2016Article
- Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification.Frontiers in plant science · 2016Article
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5 authors.
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Abstract
Acanthopanacis cortex has been used in clinical applications for a long time. Considering some historical and geographical factors, Acanthopanacis cortex is easily confused with other herbs in medicine markets, thereby causing potential safety issues. In this study, we used the internal transcribed spacer 2 (ITS2) barcode to identify 69 samples belonging to six species, including Acanthopanacis cortex and its adulterants. The nearest distance, single-nucleotide polymorphisms (SNPs), and neighbor-joining (NJ) tree methods were used to evaluate the identification ability of the ITS2 barcode. According to the kimura-2-parameter model, the intraspecific distance of Eleutherococcus nodiflorus ITS2 sequences ranged from 0 to 0.0132. The minimum interspecific distance between E. nodiflorus and E. giraldii was 0.0221, which was larger than the maximum intraspecific distance of E. nodiflorus. Three stable SNPs in ITS2 can be used to distinguish Acanthopanacis cortex and its closely related species. The NJ tree indicated that the Acanthopanacis cortex samples clustered into one clade, which can be distinguished clearly from the adulterants of this herb. A secondary structure of ITS2 provided another dimensionality to identify species. In conclusion, the ITS2 barcode effectively identifies Acanthopanacis cortex, and DNA barcoding is a convenient tool for medicine market supervision.
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