ArticleFrontiers in plant science2015
Transition from somatic embryo to friable embryogenic callus in cassava: dynamic changes in cellular structure, physiological status, and gene expression profiles.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Metabolic Profiling Using Gas Chromatography-Mass Spectrometry and Optimization of Light-Emitting Diodes for Callus Induction in Patchouli "Tapak Tuan" (Pogostemon cablin Benth.).Rapid communications in mass spectrometry : RCM · 2026Article
- Effect of gamma irradiation on proliferation and growth of friable embryogenic callus andFrontiers in plant science · 2024Article
- Transcriptomic Profiling of Embryogenic and Non-Embryogenic Callus Provides New Insight into the Nature of Recalcitrance in Cannabis.International journal of molecular sciences · 2023Article
- Coconut Callus Initiation for Cell Suspension Culture.Plants (Basel, Switzerland) · 2023Article
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- Knockdown ofInternational journal of molecular sciences · 2022Article
- Agrobacterium-mediated cassava transformation for the Asian elite variety KU50.Plant molecular biology · 2022Article
- Studies on Pure MlbFrontiers in plant science · 2022Article
- Efficient Genetic Transformation and Regeneration of a Farmer-Preferred Cassava Cultivar From Ghana.Frontiers in plant science · 2021Article
- YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process inInternational journal of molecular sciences · 2020Article
- Comparative transcriptome analysis highlights the hormone effects on somatic embryogenesis in Catalpa bungei.Plant reproduction · 2019Article
- ArabidopsisFrontiers in plant science · 2019Article
- Comparative RNA-seq based transcriptome profiling of waterlogging response in cucumber hypocotyls reveals novel insights into the de novo adventitious root primordia initiation.BMC plant biology · 2017Article
- Genome-wide transcriptome profiling ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.PloS one · 2017Article
- Loss of CMD2-mediated resistance to cassava mosaic disease in plants regenerated through somatic embryogenesis.Molecular plant pathology · 2016Article
- The potential of using biotechnology to improve cassava: a review.In vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationReview
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Friable embryogenic callus (FEC) is considered as the most suitable material for efficient genetic transformation of cassava. Heavy genotype dependence of FEC induction and amenability to somaclonal variation limits the production and maintenance of reliable FEC. Identifying key elements involved in biological processes from somatic embryos (SEs) to FEC at different stages provides critical insights for FEC improvement. Cytological observation showed a dramatic change of subcellular structures among SEs, fresh FEC (FFEC), and old FEC (OFEC). Decrease of sucrose and increase of fructose and glucose were detected in OFEC. A total of 6871 differentially expressed genes (DEGs) were identified from SEs, FFEC, and OFEC by RNA-seq. Analysis of the DEGs showed that FEC induction was accompanied by the process of dedifferentiation, whereas the epigenetics modification occurred during the continuous subculturing process. The cell structure was reconstructed, mainly including the GO terms of "cell periphery" and "external encapsulating structure"; in parallel, the internal mechanisms changed correspondingly, including the biological process of glycolysis and metabolisms of alanine, aspartate, and glutamate. The significant reduction of genomic DNA methylation in OFEC indicated altered gene expression via chromatin modification. These results indicate that the induction and long-term subculture of FEC is a complicated biological process involving changes of genome modification, gene expression, and subcellular reconstruction. The findings will be useful for improving FEC induction and maintenance from farmer-preferred cassava cultivars recalcitrant to genetic transformation, hence improving cassava through genetic engineering.
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