ArticleFrontiers in microbiology2015
Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review.Memorias do Instituto Oswaldo Cruz · 2022Pooled it
- Acanthamoeba polyphaga de novo transcriptome and its dynamics during Mimivirus infection.Scientific reports · 2024Article
- A long-term prospecting study on giant viruses in terrestrial and marine Brazilian biomes.Virology journal · 2024Article
- Cytopathic effects in Mimivirus infection: understanding the kinetics of virus-cell interaction.Memorias do Instituto Oswaldo Cruz · 2024Article
- Analysis of the Genomic Features and Evolutionary History of Pithovirus-Like Isolates Reveals Two Major Divergent Groups of Viruses.Journal of virology · 2023Article
- Diversity of Surface Fibril Patterns in Mimivirus Isolates.Journal of virology · 2023Article
- Giant virus biology and diversity in the era of genome-resolved metagenomics.Nature reviews. Microbiology · 2022Review
- The Astounding World of Glycans from Giant Viruses.Chemical reviews · 2022Review
- Viral histones: pickpocket's prize or primordial progenitor?Epigenetics & chromatin · 2022Review
- Article
- Review
- Draft Genome Sequence of Pandoravirus japonicus Isolated from the Sabaishi River, Niigata, Japan.Microbiology resource announcements · 2021Article
- Review
- Giant virus vs amoeba: fight for supremacy.Virology journal · 2019Review
- Review
- New Isolates of Pandoraviruses: Contribution to the Study of Replication Cycle Steps.Journal of virology · 2019Article
- High-throughput isolation of giant viruses using high-content screening.Communications biology · 2019Article
- Cedratvirus getuliensis replication cycle: an in-depth morphological analysis.Scientific reports · 2018Article
- Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica.Virology journal · 2018Article
- Analyses of the Kroon Virus Major Capsid Gene and Its Transcript Highlight a Distinct Pattern of Gene Evolution and Splicing among Mimiviruses.Journal of virology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Megavirales are a newly described order capable of infecting different types of eukaryotic hosts. For the most part, the natural host is unknown. Several methods have been used to detect these viruses, with large discrepancies between molecular methods and co-cultures. To isolate giant viruses, we propose the use of different species of amoeba as a cellular support. The aim of this work was to isolate new Brazilian giant viruses by comparing the protozoa Acanthamoeba castellanii, A. polyphaga, A. griffini, and Vermamoeba vermiformis (VV) as a platform for cellular isolation using environmental samples. One hundred samples were collected from 3 different areas in September 2014 in the Pampulha lagoon of Belo Horizonte city, Minas Gerais, Brazil. PCR was used to identify the isolated viruses, along with hemacolor staining, labelling fluorescence and electron microscopy. A total of 69 viruses were isolated. The highest ratio of isolation was found in A. polyphaga (46.38%) and the lowest in VV (0%). Mimiviruses were the most frequently isolated. One Marseillevirus and one Pandoravirus were also isolated. With Brazilian environmental samples, we demonstrated the high rate of lineage A mimiviruses. This work demonstrates how these viruses survive and circulate in nature as well the differences between protozoa as a platform for cellular isolation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.