ArticleMolecular biology and evolution2016
First-Step Mutations during Adaptation Restore the Expression of Hundreds of Genes.
Article in Molecular biology and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 113 citations in OpenAlex.
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- Using TraDIS as a complementary approach to long term evolution for mapping adaptive mutations in Escherichia coli.PLoS genetics · 2026Article
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- Repeatability of gene expression evolution in experimental environmental adaptation.Nature communications · 2026Article
- Enhancing the heat tolerance of homolactic Escherichia coli through reverse metabolic engineering.Applied microbiology and biotechnology · 2026Article
- Environmental stress shapes persistence-like phenotypes and genomic changes inFrontiers in microbiology · 2026Article
- Molecular frontiers in hemodialysis: unraveling the role of membranes in gene expression, epigenetics, and inflammatory pathways.International urology and nephrology · 2025Review
- Thermally adapted Escherichia coli keeps transcriptomic response during temperature upshift exposure.Applied microbiology and biotechnology · 2025Article
- Distinguishing among evolutionary and ecological processes shaping microbiome dynamics.The ISME journal · 2025Review
- Thermal Plasticity and Evolutionary Constraints inBiology · 2024Article
- Genome and transcriptomic analysis of the adaptation ofComputational and structural biotechnology journal · 2024Review
- Microbial diversification is maintained in an experimentally evolved synthetic community.mSystems · 2024Article
- Patterns of Fitness and Gene Expression Epistasis Generated by Beneficial Mutations in the rho and rpoB Genes of Escherichia coli during High-Temperature Adaptation.Molecular biology and evolution · 2024Article
- Experimental Evolution in a Warming World: The Omics Era.Molecular biology and evolution · 2024Review
- Genotype-by-environment interactions govern fitness changes associated with adaptive mutations in two-component response systems.Frontiers in genetics · 2024Article
- Convergent genetic adaptation ofFrontiers in molecular biosciences · 2024Article
- Development of heat-shock resistance inApplied and environmental microbiology · 2023Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The temporal change of phenotypes during the adaptive process remains largely unexplored, as do the genetic changes that affect these phenotypic changes. Here we focused on three mutations that rose to high frequency in the early stages of adaptation within 12 Escherichia coli populations subjected to thermal stress (42 °C). All the mutations were in the rpoB gene, which encodes the RNA polymerase beta subunit. For each mutation, we measured the growth curves and gene expression (mRNAseq) of clones at 42 °C. We also compared growth and gene expression with their ancestor under unstressed (37 °C) and stressed conditions (42 °C). Each of the three mutations changed the expression of hundreds of genes and conferred large fitness advantages, apparently through the restoration of global gene expression from the stressed toward the prestressed state. These three mutations had a similar effect on gene expression as another single mutation in a distinct domain of the rpoB protein. Finally, we compared the phenotypic characteristics of one mutant, I572L, with two high-temperature adapted clones that have this mutation plus additional background mutations. The background mutations increased fitness, but they did not substantially change gene expression. We conclude that early mutations in a global transcriptional regulator cause extensive changes in gene expression, many of which are likely under positive selection for their effect in restoring the prestress physiology.
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