ArticleScientific reports2015
Efficient synthesis of stably adenylated DNA and RNA adapters for microRNA capture using T4 RNA ligase 1.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Draft genome sequence of cadmium-tolerantMicrobiology resource announcements · 2026Article
- Cancer mutations rewire the RNA methylation specificity of METTL3-METTL14.Science advances · 2024Article
- Formamide significantly enhances the efficiency of chemical adenylation of RNA sequencing ligation adaptors.RNA (New York, N.Y.) · 2023Article
- Article
- Direct adenylation from 5'-OH-terminated oligonucleotides by a fusion enzyme containing Pfu RNA ligase and T4 polynucleotide kinase.Nucleic acids research · 2022Article
- Strategies and Best Practice in Cloning Small RNAs.Gene technology · 2020Article
- Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA.Nucleic acids research · 2018Article
- Article
- Capture and sequencing of NAD-capped RNA sequences with NAD captureSeq.Nature protocols · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
MicroRNA profiling methods have become increasingly important due to the rapid rise of microRNA in both basic and translational sciences. A critical step in many microRNA profiling assays is adapter ligation using pre-adenylated adapters. While pre-adenylated adapters can be chemically or enzymatically prepared, enzymatic adenylation is preferred due to its ease and high yield. However, previously reported enzymatic methods either require tedious purification steps or use thermostable ligases that can generate side products during the subsequent ligation step. We have developed a highly efficient, template- and purification-free, adapter adenylation method using T4 RNA ligase 1. This method is capable of adenylating large amounts of adapter at ~100% efficiency and can efficiently adenylate both DNA and RNA bases. We find that the adenylation reaction speed can differ between DNA and RNA and between terminal nucleotides, leading to bias if reactions are not allowed to run to completion. We further find that the addition of high PEG levels can effectively suppress these differences.
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Registered trials
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