ArticleMicroscopy research and technique2015
Modulated CMOS camera for fluorescence lifetime microscopy.
Article in Microscopy research and technique, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Phasor EO-FLIM: Lifetime imaging with picosecond noise and 500 Hz frame rate.bioRxiv : the preprint server for biology · 2026Article
- Low-Energy Single-Electron Detector with Submicron Resolution.ACS photonics · 2026Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Periodic Light Modulations for Low-Cost Wide-Field Imaging of Luminescence Kinetics Under Ambient Light.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Detection of a Water-Soluble Hypericin Formulation in Glioblastoma Tissue with Fluorescence Lifetime and Intensity Using a Dual-Tap CMOS Camera System.Diagnostics (Basel, Switzerland) · 2024Article
- Novel Approach for Lifetime-Proportional Luminescence Imaging Using Frame Straddling.ACS sensors · 2024Article
- Implementation of FRET Spectrometry Using Temporally Resolved Fluorescence: A Feasibility Study.International journal of molecular sciences · 2024Article
- Article
- Label-free fluorescence microscopy: revisiting the opportunities with autofluorescent molecules and harmonic generations as biosensors and biomarkers for quantitative biology.Biophysical reviews · 2023Review
- Comprehensive Investigation of Parameters Influencing Fluorescence Lifetime Imaging Microscopy in Frequency- and Time-Domain Illustrated by Phasor Plot Analysis.International journal of molecular sciences · 2022Article
- Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems.Cell reports methods · 2022Article
- Photophysical properties and fluorescence lifetime imaging of exfoliated near-infrared fluorescent silicate nanosheets.Nanoscale advances · 2021Article
- Full-field fluorescence lifetime dual-comb microscopy using spectral mapping and frequency multiplexing of dual-comb optical beats.Science advances · 2021Article
- Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.Journal of biomedical optics · 2020Review
- Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots.Nanomaterials (Basel, Switzerland) · 2020Article
- Towards real-time wide-field fluorescence lifetime imaging of 5-ALA labeled brain tumors with multi-tap CMOS cameras.Biomedical optics express · 2020Article
- High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.Methods and applications in fluorescence · 2020Review
- Auto-Phase-Locked Time-Resolved Luminescence Detection: Principles, Applications, and Prospects.Frontiers in chemistry · 2020Review
- Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy.Nature communications · 2019Article
- Fast single-cell biochemistry: theory, open source microscopy and applications.Methods and applications in fluorescence · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Widefield frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) is a fast and accurate method to measure the fluorescence lifetime of entire images. However, the complexity and high costs involved in construction of such a system limit the extensive use of this technique. PCO AG recently released the first luminescence lifetime imaging camera based on a high frequency modulated CMOS image sensor, QMFLIM2. Here we tested and provide operational procedures to calibrate the camera and to improve the accuracy using corrections necessary for image analysis. With its flexible input/output options, we are able to use a modulated laser diode or a 20 MHz pulsed white supercontinuum laser as the light source. The output of the camera consists of a stack of modulated images that can be analyzed by the SimFCS software using the phasor approach. The nonuniform system response across the image sensor must be calibrated at the pixel level. This pixel calibration is crucial and needed for every camera settings, e.g. modulation frequency and exposure time. A significant dependency of the modulation signal on the intensity was also observed and hence an additional calibration is needed for each pixel depending on the pixel intensity level. These corrections are important not only for the fundamental frequency, but also for the higher harmonics when using the pulsed supercontinuum laser. With these post data acquisition corrections, the PCO CMOS-FLIM camera can be used for various biomedical applications requiring a large frame and high speed acquisition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.