ArticleeLife2015
Self-establishing communities enable cooperative metabolite exchange in a eukaryote.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Low initial metabolite production enhances stability in syntrophic bacterial consortia.Communications biology · 2026Article
- De novo biosynthesis of plant lignans by synthetic yeast consortia.Nature chemical biology · 2025Article
- A dual reporter system for intracellular and extracellular amino acid sensing in budding yeast.Molecular biology of the cell · 2025Article
- Review
- Asymmetric metabolic adaptations undermine stability in microbial syntrophy.ISME communications · 2025Article
- Engineered cross-feeding creates inter- and intra-species synthetic yeast communities with enhanced bioproduction.Nature communications · 2024Article
- The evolution of autonomy from two cooperative specialists in fluctuating environments.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities.Nature microbiology · 2024Article
- Cross-feeding promotes heterogeneity within yeast cell populations.Nature communications · 2024Article
- Metabolic exchanges are ubiquitous in natural microbial communities.Nature microbiology · 2023Review
- Membrane transporters in cell physiology, cancer metabolism and drug response.Disease models & mechanisms · 2023Review
- Spontaneously established syntrophic yeast communities improve bioproduction.Nature chemical biology · 2023Article
- Article
- Unravelling metabolic cross-feeding in a yeast-bacteria community usingMolecular systems biology · 2023Article
- Impact of direct physical association and motility on fitness of a synthetic interkingdom microbial community.The ISME journal · 2023Article
- Metabolic heterogeneity and cross-feeding within isogenic yeast populations captured by DILAC.Nature microbiology · 2023Article
- Article
- Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy.PLoS biology · 2022Article
- Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant Saccharomyces cerevisiae under Glucose-Limited Conditions.Applied and environmental microbiology · 2022Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Metabolite exchange among co-growing cells is frequent by nature, however, is not necessarily occurring at growth-relevant quantities indicative of non-cell-autonomous metabolic function. Complementary auxotrophs of Saccharomyces cerevisiae amino acid and nucleotide metabolism regularly fail to compensate for each other's deficiencies upon co-culturing, a situation which implied the absence of growth-relevant metabolite exchange interactions. Contrastingly, we find that yeast colonies maintain a rich exometabolome and that cells prefer the uptake of extracellular metabolites over self-synthesis, indicators of ongoing metabolite exchange. We conceived a system that circumvents co-culturing and begins with a self-supporting cell that grows autonomously into a heterogeneous community, only able to survive by exchanging histidine, leucine, uracil, and methionine. Compensating for the progressive loss of prototrophy, self-establishing communities successfully obtained an auxotrophic composition in a nutrition-dependent manner, maintaining a wild-type like exometabolome, growth parameters, and cell viability. Yeast, as a eukaryotic model, thus possesses extensive capacity for growth-relevant metabolite exchange and readily cooperates in metabolism within progressively establishing communities.
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