ArticleBMC plant biology2015
Monocot and dicot MLO powdery mildew susceptibility factors are functionally conserved in spite of the evolution of class-specific molecular features.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 65 citations in OpenAlex.
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- Genetic engineering, including genome editing, for enhancing broad-spectrum disease resistance in crops.Plant communications · 2025Review
- The First Genome-Wide Mildew Locus O Genes Characterization in theInternational journal of molecular sciences · 2023Article
- Functional and evolutionary study of MLO gene family in the regulation of Sclerotinia stem rot resistance in Brassica napus L.Biotechnology for biofuels and bioproducts · 2023Article
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- CRISPR/Cas9 basedFrontiers in plant science · 2022Article
- Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Evolution of the MLO gene families in octoploid strawberry (Fragaria ×ananassa) and progenitor diploid species identified potential genes for strawberry powdery mildew resistance.Horticulture research · 2021Article
- Discovery and Characterization of a Novel TomatoGenes · 2021Article
- Screening of CRISPR/Cas9 gRNA for mimicking Powdery Mildew resistant MLO ol-2 mutant.Bioinformation · 2021Article
- Phylogenetic Relationship of PlantGenes · 2020Article
- Comparative transcriptome analysis uncovers regulatory roles of long non-coding RNAs involved in resistance to powdery mildew in melon.BMC genomics · 2020Article
- Interaction Between AtCML9 and AtMLO10 Regulates Pollen Tube Development and Seed Setting.Frontiers in plant science · 2020Article
- Arabidopsis mlo3 mutant plants exhibit spontaneous callose deposition and signs of early leaf senescence.Plant molecular biology · 2019Article
- Evolutionary conservation of MLO gene promoter signatures.BMC plant biology · 2019Article
- siRNA-Finder (si-Fi) Software for RNAi-Target Design and Off-Target Prediction.Frontiers in plant science · 2019Article
- Article
- Functional characterization of the powdery mildew susceptibility gene SmMLO1 in eggplant (Solanum melongena L.).Transgenic research · 2017Article
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpecific members of the plant Mildew Locus O (MLO) protein family act as susceptibility factors towards powdery mildew (PM), a worldwide-spread fungal disease threatening many cultivated species. Previous studies indicated that monocot and dicot MLO susceptibility proteins are phylogenetically divergent.
methodsA bioinformatic approach was followed to study the type of evolution of Angiosperm MLO susceptibility proteins. Transgenic complementation tests were performed for functional analysis.
resultsOur results show that monocot and dicot MLO susceptibility proteins evolved class-specific conservation patterns. Many of them appear to be the result of negative selection and thus are likely to provide an adaptive value. We also tested whether different molecular features between monocot and dicot MLO proteins are specifically required by PM fungal species to cause pathogenesis. To this aim, we transformed a tomato mutant impaired for the endogenous SlMLO1 gene, and therefore resistant to the tomato PM species Oidium neolycopersici, with heterologous MLO susceptibility genes from the monocot barley and the dicot pea. In both cases, we observed restoration of PM symptoms. Finally, through histological observations, we demonstrate that both monocot and dicot susceptibility alleles of the MLO genes predispose to penetration of a non-adapted PM fungal species in plant epidermal cells.
conclusionsWith this study, we provide insights on the evolution and function of MLO genes involved in the interaction with PM fungi. With respect to breeding research, we show that transgenic complementation assays involving phylogenetically distant plant species can be used for the characterization of novel MLO susceptibility genes. Moreover, we provide an overview of MLO protein molecular features predicted to play a major role in PM susceptibility. These represent ideal targets for future approaches of reverse genetics, addressed to the selection of loss-of-function resistant mutants in cultivated species.
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