ArticleBMC genomics2015
Transcriptome analysis of Brassica napus pod using RNA-Seq and identification of lipid-related candidate genes.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach.BMC plant biology · 2020Pooled it
- Transcriptome analysis identifies genes regulating self-compatibility, flowering time, and oil biosynthesis in Noug (Guizotia abyssinica).Scientific reports · 2025Article
- Fine mapping and candidate gene analysis of a major QTL for oil content in the seed of Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops.International journal of molecular sciences · 2022Review
- Global transcriptome analysis reveals potential genes associated with genic male sterility of rapeseed (Frontiers in plant science · 2022Article
- Transcriptome Analysis of Seed Weight Plasticity inInternational journal of molecular sciences · 2021Article
- Comparative analysis of sugarcane root transcriptome in response to the plant growth-promoting Burkholderia anthina MYSP113.PloS one · 2020Article
- Single-molecule real-time sequencing facilitates the analysis of transcripts and splice isoforms of anthers in Chinese cabbage (Brassica rapa L. ssp. pekinensis).BMC plant biology · 2019Article
- Protective Role of Leaf Variegation inInternational journal of molecular sciences · 2019Article
- Unravelling the MicroRNA-Mediated Gene Regulation in Developing Pongamia Seeds by High-Throughput Small RNA Profiling.International journal of molecular sciences · 2019Article
- De novo sequencing of tree peony (Paeonia suffruticosa) transcriptome to identify critical genes involved in flowering and floral organ development.BMC genomics · 2019Article
- Overexpression of Acyl-ACP Thioesterases,International journal of molecular sciences · 2019Article
- Comparative transcriptomic analysis of high- and low-oil3 Biotech · 2019Article
- Dynamic Transcriptome Changes Related to Oil Accumulation in Developing Soybean Seeds.International journal of molecular sciences · 2019Article
- Comparative Transcriptome Analysis between Fertile and CMS Flower Buds in Wucai (Brassica campestris L.).BMC genomics · 2018Article
- An integrated omics analysis reveals molecular mechanisms that are associated with differences in seed oil content between Glycine max and Brassica napus.BMC plant biology · 2018Article
- Cloning and characterization of EgGDSL, a gene associated with oil content in oil palm.Scientific reports · 2018Article
- Temporal transcriptome profiling of developing seeds reveals a concerted gene regulation in relation to oil accumulation in Pongamia (Millettia pinnata).BMC plant biology · 2018Article
- Article
- De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments.International journal of molecular sciences · 2017Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundBrassica napus is an important oilseed crop. Dissection of the genetic architecture underlying oil-related biological processes will greatly facilitates the genetic improvement of rapeseed. The differential gene expression during pod development offers a snapshot on the genes responsible for oil accumulation in. To identify candidate genes in the linkage peaks reported previously, we used RNA sequencing (RNA-Seq) technology to analyze the pod transcriptomes of German cultivar Sollux and Chinese inbred line Gaoyou.
methodsThe RNA samples were collected for RNA-Seq at 5-7, 15-17 and 25-27 days after flowering (DAF). Bioinformatics analysis was performed to investigate differentially expressed genes (DEGs). Gene annotation analysis was integrated with QTL mapping and Brassica napus pod transcriptome profiling to detect potential candidate genes in oilseed.
resultsFour hundred sixty five and two thousand, one hundred fourteen candidate DEGs were identified, respectively, between two varieties at the same stages and across different periods of each variety. Then, 33 DEGs between Sollux and Gaoyou were identified as the candidate genes affecting seed oil content by combining those DEGs with the quantitative trait locus (QTL) mapping results, of which, one was found to be homologous to Arabidopsis thaliana lipid-related genes. DISCUSSION: Intervarietal DEGs of lipid pathways in QTL regions represent important candidate genes for oil-related traits. Integrated analysis of transcriptome profiling, QTL mapping and comparative genomics with other relative species leads to efficient identification of most plausible functional genes underlying oil-content related characters, offering valuable resources for bettering breeding program of Brassica napus.
conclusionsThis study provided a comprehensive overview on the pod transcriptomes of two varieties with different oil-contents at the three developmental stages.
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