ArticleParasitology research2016
Evaluation of the basic functions of six calcium-dependent protein kinases in Toxoplasma gondii using CRISPR-Cas9 system.
Article in Parasitology research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 41 citations in OpenAlex.
- Calcium-dependent protein kinases 2A involved in the growth of both asexual and sexual stages of Cryptosporidium parvum.PLoS neglected tropical diseases · 2025Article
- Considerations on Structural Vaccinology and Epitope Screening of Calcium-Dependent Protein Kinases 8 as a Potential Vaccine Target AgainstInterdisciplinary perspectives on infectious diseases · 2025Article
- Functional Characterization of Eight Zinc Finger Motif-Containing Proteins inPathogens (Basel, Switzerland) · 2023Article
- Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System.Microbiology spectrum · 2023Article
- Virulence-related gene wx2 of Toxoplasma gondii regulated host immune response via classic pyroptosis pathway.Parasites & vectors · 2022Article
- Live-attenuated ME49Δcdpk3 strain of Toxoplasma gondii protects against acute and chronic toxoplasmosis.NPJ vaccines · 2022Article
- CRISPR-Cas Technology: Emerging Applications in Clinical Microbiology and Infectious Diseases.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Characterization of functions in parasite growth and virulence of four Toxoplasma gondii genes involved in lipid synthesis by CRISPR-Cas9 system.Parasitology research · 2021Article
- Effect of deletion of gra17 and gra23 genes on the growth, virulence, and immunogenicity of type II Toxoplasma gondii.Parasitology research · 2020Article
- A NovelFrontiers in microbiology · 2020Article
- Study on Circulating Antigens in Serum of Mice With Experimental Acute Toxoplasmosis.Frontiers in microbiology · 2020Article
- Functional Characterization of Two Thioredoxin Proteins ofFrontiers in veterinary science · 2020Article
- Characterization of Three Calcium-Dependent Protein Kinases ofFrontiers in microbiology · 2020Article
- Characterization of Two EF-hand Domain-containing Proteins from Toxoplasma gondii.The Journal of eukaryotic microbiology · 2019Article
- Characterization of the Role of Amylo-Alpha-1,6-Glucosidase Protein in the Infectivity ofFrontiers in cellular and infection microbiology · 2019Article
- Immunization With a Live-Attenuated RH:ΔFrontiers in microbiology · 2019Article
- New and emerging uses of CRISPR/Cas9 to genetically manipulate apicomplexan parasites.Parasitology · 2018Review
- The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites.Applied microbiology and biotechnology · 2018Review
- Microbial protein targets: towards understanding and intervention.Parasitology · 2018Article
- The calcium-dependent protein kinase 1 from Toxoplasma gondii as target for structure-based drug design.Parasitology · 2018Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxoplasma gondii, an important protozoan parasite, infects almost all warm-blooded animals and humans. Although treatments in T. gondii are limited by the lack of effective drugs, some calcium-dependent kinases were demonstrated as the promising drug targets to chemotherapy against T. gondii due to their essential roles in T. gondii and absence from their hosts. The objectives of the present study were to investigate the functions of six calcium-dependent protein kinases (CDPK4, CDPK4A, CDPK5, CDPK6, CDPK8, and CDPK9) in T. gondii to assess whether they are suitable for designing as drug targets. We used the CRISPR-Cas9 system to disrupt six CDPK genes successfully by insertion of DHFR* at the guide RNA-targeted region in the six endogenous CDPK loci and successfully obtained the six knockout (KO)-CDPK strains. The biological characteristics of the six strains were evaluated by plaque assays, invasion, egress, replication, and virulence assays, respectively. The results indicated that there was no significant difference between the six KO-CDPK strains and wild-type strain in virulence and the lytic cycle including invasion, egress, and replication. The conclusion was the six CDPKs are not essential for T. gondii lytic cycle and also not virulence factors for mice, suggesting that the six CDPKs may participate in other functions in T. gondii.
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