ArticleScientific reports2015
Interaction between PVY HC-Pro and the NtCF1β-subunit reduces the amount of chloroplast ATP synthase in virus-infected tobacco.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 26 citations in OpenAlex.
- Isobavachalcone Alleviates Plant Photosynthesis Inhibition Caused by Tobacco Mosaic Virus (TMV) Infection in Tobacco.Plants (Basel, Switzerland) · 2025Article
- A Comprehensive Screening of the Interactors of Areca Palm Necrotic Ringspot Virus (ANRSV) HCPro2 Highlights the Proviral Roles of eIF4A and PGK in Viral Infection.Plants (Basel, Switzerland) · 2025Article
- Comprehensive proteomic profiling of Cucumber mosaic virus infection: identifying key proteins and pathways involved in resistance and susceptibility in melon.BMC plant biology · 2025Article
- Viral coat proteins decrease the gene silencing activity of cognate and heterologous viral suppressors.Scientific reports · 2024Article
- Research Progress on Viruses ofBiology · 2024Review
- Potyviral Helper-Component Protease: Multifaced Functions and Interactions with Host Proteins.Plants (Basel, Switzerland) · 2024Review
- Unraveling the Mechanisms of Virus-Induced Symptom Development in Plants.Plants (Basel, Switzerland) · 2023Review
- Transcriptome sequencing and functional verification revealed the roles of exogenous magnesium in tobacco anti-PVY infection.Frontiers in microbiology · 2023Article
- A maize triacylglycerol lipase inhibits sugarcane mosaic virus infection.Plant physiology · 2022Article
- Photosynthesis-related genes induce resistance against soybean mosaic virus: Evidence for involvement of the RNA silencing pathway.Molecular plant pathology · 2022Article
- Positive Selection in the Chloroplastic ATP-Synthase β-Subunit and Its Relation to Virulence Factors.Journal of molecular evolution · 2020Article
- Characterization of Proteins Involved in Chloroplast Targeting Disturbed by Rice Stripe Virus by Novel Protoplast⁻Chloroplast Proteomics.International journal of molecular sciences · 2019Article
- The HCPro from the Potyviridae family: an enviable multitasking Helper Component that every virus would like to have.Molecular plant pathology · 2018Review
- Inducible transgenic tobacco system to study the mechanisms underlying chlorosis mediated by the silencing of chloroplast heat shock protein 90.Virusdisease · 2017Article
- Chloroplast in Plant-Virus Interaction.Frontiers in microbiology · 2016Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The photosynthetic rate of virus-infected plants is always reduced. However, the molecular mechanism underlying this phenomenon remains unclear. The helper component-proteinase (HC-Pro) of Potato virus Y (PVY) was found in the chloroplasts of PVY-infected tobacco, indicating some new function of HC-Pro in the chloroplasts. We generated HC-Pro transgenic plants with a transit peptide to target the protein to chloroplast. The HC-Pro transgenic tobacco showed a decreased photosynthetic rate by 25% at the light intensity of 600 μmol m(-2) s(-1). Using a yeast two-hybrid screening assay to search for chloroplast proteins interacting with HC-Pro, we identified that PVY HC-Pro can interact with the chloroplast ATP synthase NtCF1β-subunit. This interaction was confirmed by GST pull-down and co-immunoprecipitation assays. HC-Pro didn't interfere with the activity of assembled ATP synthase in vitro. The HC-Pro/NtCF1β-subunit interaction might affect the assembly of ATP synthase complex. Quantitative western blot and immunogold labeling of the ATP synthase indicated that the amount of ATP synthase complex was decreased in both the HC-Pro transgenic and the PVY-infected tobacco. These results demonstrate that HC-Pro plays an important role in reducing the photosynthetic rate of PVY-infected plants, which is a completely new role of HC-Pro besides its multiple known functions.
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