ArticleExtremophiles : life under extreme conditions2016
A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering.
Article in Extremophiles : life under extreme conditions, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Alkylated DNA repair by a novel HhH-GPD family protein from Crenarchaea.Nucleic acids research · 2025Article
- Archaeal DNA alkylation repair conducted by DNA glycosylase and methyltransferase.Applied microbiology and biotechnology · 2023Review
- A fluorescent reporter system for anaerobic thermophiles.Frontiers in bioengineering and biotechnology · 2023Article
- First thermostable CLIP-Computational and structural biotechnology journal · 2022Article
- The SNAP-Journal of enzyme inhibition and medicinal chemistry · 2021Article
- Folding-upon-Repair DNA Nanoswitches for Monitoring the Activity of DNA Repair Enzymes.Angewandte Chemie (International ed. in English) · 2021Article
- Review
- A journey down to hell: new thermostable protein-tags for biotechnology at high temperatures.Extremophiles : life under extreme conditions · 2020Article
- An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane.Journal of enzyme inhibition and medicinal chemistry · 2019Article
- Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLJournal of enzyme inhibition and medicinal chemistry · 2019Article
- Optimization of an In Vitro Transcription/Translation System Based onArchaea (Vancouver, B.C.) · 2019Article
- Every OGT Is Illuminated … by Fluorescent and Synchrotron Lights.International journal of molecular sciences · 2017Review
- In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag.PloS one · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the last decade, a powerful biotechnological tool for the in vivo and in vitro specific labeling of proteins (SNAP-tag™ technology) was proposed as a valid alternative to classical protein-tags (green fluorescent proteins, GFPs). This was made possible by the discovery of the irreversible reaction of the human alkylguanine-DNA-alkyl-transferase (hAGT) in the presence of benzyl-guanine derivatives. However, the mild reaction conditions and the general instability of the mesophilic SNAP-tag™ make this new approach not fully applicable to (hyper-)thermophilic and, in general, extremophilic organisms. Here, we introduce an engineered variant of the thermostable alkylguanine-DNA-alkyl-transferase from the Archaea Sulfolobus solfataricus (SsOGT-H5), which displays a catalytic efficiency comparable to the SNAP-tag™ protein, but showing high intrinsic stability typical of proteins from this organism. The successful heterologous expression obtained in a thermophilic model organism makes SsOGT-H5 a valid candidate as protein-tag for organisms living in extreme environments.
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