ArticleEnvironmental science and pollution research international2017
Pushing the limits of nickel detection to nanomolar range using a set of engineered bioluminescent Escherichia coli.
Article in Environmental science and pollution research international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Advances and perspectives in genetically encoded biosensors for biomarker detection and theranostic circuits.Synthetic and systems biotechnology · 2027Review
- Engineering Allosteric Transcription Factor-Based Biosensors: Advances and Prospects for Modern Food Contaminant Monitoring.Foods (Basel, Switzerland) · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Detection of environmental pollutants using transcription factor-based whole-cell biosensors.Biotechnology for the environment · 2025Review
- Bacterial degrons in synthetic circuits.Open biology · 2022Review
- Use of whole-cell bioreporters to assess bioavailability of contaminants in aquatic systems.Frontiers in chemistry · 2022Review
- Increased sensitivity of heavy metal bioreporters in transporter deficient Synechocystis PCC6803 mutants.PloS one · 2021Article
- Synthetic Biology Enables Programmable Cell-Based Biosensors.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Review
- Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences.Frontiers in microbiology · 2020Review
- The Application of Whole Cell-Based Biosensors for Use in Environmental Analysis and in Medical Diagnostics.Sensors (Basel, Switzerland) · 2017Review
- Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors.Environmental science and pollution research international · 2017Article
- Semi-autonomous inline water analyzer: design of a common light detector for bacterial, phage, and immunological biosensors.Environmental science and pollution research international · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection of nickel in water is of great importance due to its harmfulness for living organism. A way to detect Ni is the use of whole-cell biosensors. The aim of the present work was to build a light-emitting bacterial biosensor for the detection of Ni with high specificity and low detection limit properties. For that purpose, the regulatory circuit implemented relied on the RcnR Ni/Co metallo-regulator and its rcnA natural target promoter fused to the lux reporter genes. To convert RcnR to specifically detect Ni, several mutations were tested and the C35A retained. Deleting the Ni efflux pump rcnA and introducing genes encoding several Ni-uptake systems lowered the detection thresholds. When these constructs were assayed in several Escherichia coli strains, it appeared that the detection thresholds were highly variable. The TD2158 wild-type E. coli gave rise to a biosensor ten times more active and sensitive than its W3110 E. coli K12 equivalent. This biosensor was able to confidently detect Ni concentrations as little as 80 nM (4.7 μg l
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