ArticleMethods in molecular biology (Clifton, N.J.)2016
Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging.
Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques.Plant methods · 2026Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Differentiation Disorders ofCells · 2023Article
- Article
- Laticifer growth pattern is guided by cytoskeleton organization.Frontiers in plant science · 2022Article
- Cortical tension overrides geometrical cues to orient microtubules in confined protoplasts.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- A new kymogram-based method reveals unexpected effects of marker protein expression and spatial anisotropy of cytoskeletal dynamics in plant cell cortex.Plant methods · 2017Article
- An improved immunofluorescence staining method for chloroplast proteins.Plant cell reports · 2016Article
- The impact of mechanical compression on cortical microtubules in Arabidopsis: a quantitative pipeline.The Plant journal : for cell and molecular biology · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Microtubules (MTs) are required throughout plant development for a wide variety of processes, and different strategies have evolved to visualize and analyze them. This chapter provides specific methods that can be used to analyze microtubule organization and dynamic properties in plant systems and summarizes the advantages and limitations for each technique. We outline basic methods for preparing samples for immunofluorescence labeling, including an enzyme-based permeabilization method, and a freeze-shattering method, which generates microfractures in the cell wall to provide antibodies access to cells in cuticle-laden aerial organs such as leaves. We discuss current options for live cell imaging of MTs with fluorescently tagged proteins (FPs), and provide chemical fixation, high-pressure freezing/freeze substitution, and post-fixation staining protocols for preserving MTs for transmission electron microscopy and tomography.
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Registered trials
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