Evidence map›Paper›PMID 26498778›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast.

Ryo Higuchi-Sanabria, Theresa C Swayne, Istvan R Boldogh, Liza A Pon

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Imaging of Actin Cytoskeletal Integrity During Aging in C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ryo Higuchi-SanabriaDepartment of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, 630 W. 168th Street, New York, NY, 10032, USA.
Theresa C SwayneConfocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA.
Istvan R BoldoghDepartment of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, 630 W. 168th Street, New York, NY, 10032, USA.
Liza A PonDepartment of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, 630 W. 168th Street, New York, NY, 10032, USA. lap5@cumc.columbia.edu.

Funding

Tumor Biology and Microenvironment ProgramP30CA013696 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Parisa Tehranifar · 1985 to 2026
$115.3M
MITOCHONDRIAL MOTILITY AND INHERITANCER01GM045735 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI PON, LIZA A · 1993 to 2016
$4.2M
Mitochondrial-cytoskeletal interactions and agingR01GM096445 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI PON, LIZA A · 2011 to 2014
$1.3M
Super-resolution structured illumination microscopeS10OD014584 · OD · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI PON, LIZA A · 2013 to 2013
$579k
NCI NIH HHS 5 P30 CA13696NIGMS NIH HHS GM096445NIGMS NIH HHS GM45735NIGMS NIH HHS GM45735S1NIGMS NIH HHS R01 GM045735NIGMS NIH HHS R01 GM096445NIH HHS 1S10OD014584
6 · The paper itself

Abstract

Maintenance and regulation of proper mitochondrial dynamics and functions are necessary for cellular homeostasis. Numerous diseases, including neurodegeneration and muscle myopathies, and overall cellular aging are marked by declining mitochondrial function and subsequent loss of multiple other cellular functions. For these reasons, optimized protocols are needed for visualization and quantification of mitochondria and their function and fitness. In budding yeast, mitochondria are intimately associated with the actin cytoskeleton and utilize actin for their movement and inheritance. This chapter describes optimal approaches for labeling mitochondria and the actin cytoskeleton in living budding yeast cells, for imaging the labeled cells, and for analyzing the resulting images.

Indexed as

AcetatesActin CytoskeletonChromosomes, FungalColoring AgentsGenetic LociGenetic VectorsIntegrasesMitochondriaMolecular ImagingMovementOptical ImagingPolymerase Chain ReactionSaccharomycetalesSequence AnalysisTransformation, GeneticAcetatesColoring AgentsCre recombinaseIntegraseslithium acetateActinCytoskeletonDeconvolutionFluorescent proteinsLive-cell imagingMicroscopyMitochondriaRatio imagingVital stainingYeast

Identifiers

PMID26498778
PMCPMC8522908

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.