ArticlePflugers Archiv : European journal of physiology2016
Corticomedullary difference in the effects of dietary Ca²⁺ on tight junction properties in thick ascending limbs of Henle's loop.
Article in Pflugers Archiv : European journal of physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Inhibition of sodium/hydrogen exchanger 3 in the gastrointestinal tract by tenapanor reduces paracellular phosphate permeability.Science translational medicine · 2018Trial
- Control of renal calcium permeability via a tight junctional claudin switch.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Distinct cell types along thick ascending limb express pathways for monovalent and divalent cation transport.JCI insight · 2025Article
- Calcium-Sensing Receptor in the Thick Ascending Limb and Renal Response to Hypercalcemia.Journal of the American Society of Nephrology : JASN · 2025Article
- Characterization of ROMK cellular heterogeneity along the mouse kidney thick ascending limb.Pflugers Archiv : European journal of physiology · 2025Article
- HELIX Syndrome, a Claudinopathy with Relevant Dermatological Manifestations: Report of Two New Cases.Genes · 2024Article
- Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells.International journal of molecular sciences · 2024Article
- Maternal Epidermal Growth Factor Promotes Neonatal Claudin-2 Dependent Increases in Small Intestinal Calcium Permeability.Function (Oxford, England) · 2023Article
- Pathophysiological aspects of the thick ascending limb and novel genetic defects: HELIX syndrome and transient antenatal Bartter syndrome.Pediatric nephrology (Berlin, Germany) · 2022Review
- Claudin-2 and claudin-12 form independent, complementary pores required to maintain calcium homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The role of calcium-sensing receptor signaling in regulating transepithelial calcium transport.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Activation of the calcium sensing receptor increases claudin-14 expression via a PLC -p38-Sp1 pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Differential localization patterns of claudin 10, 16, and 19 in human, mouse, and rat renal tubular epithelia.American journal of physiology. Renal physiology · 2021Article
- Claudin-2 deficiency associates with hypercalciuria in mice and human kidney stone disease.The Journal of clinical investigation · 2020Article
- Claudin-12 Knockout Mice Demonstrate Reduced Proximal Tubule Calcium Permeability.International journal of molecular sciences · 2020Article
- Claudins in Renal Physiology and Pathology.Genes · 2020Review
- Viewing Cortical Collecting Duct Function Through Phenotype-guided Single-Tubule Proteomics.Function (Oxford, England) · 2020Article
- A Novel Claudinopathy Based on Claudin-10 Mutations.International journal of molecular sciences · 2019Review
- Claudin 19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells.International journal of molecular sciences · 2019Article
- Activation of the calcium sensing receptor attenuates TRPV6-dependent intestinal calcium absorption.JCI insight · 2019Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
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Abstract
The thick ascending limb of Henle's loop (TAL) drives an important part of the reabsorption of divalent cations. This reabsorption occurs via the paracellular pathway formed by the tight junction (TJ), which in the TAL shows cation selectivity. Claudins, a family of TJ proteins, determine the permeability and selectivity of this pathway. Mice were fed with normal or high-Ca(2+) diet, and effects on the reabsorptive properties of cortical and medullary TAL segments were analysed by tubule microdissection and microperfusion. Claudin expression was investigated by immunostaining and quantitative PCR. We show that the TAL adapted to high Ca(2+) load in a sub-segment-specific manner. In medullary TAL, transcellular NaCl transport was attenuated. The transepithelial voltage decreased from 10.9 ± 0.6 mV at control diet to 8.3 ± 0.5 mV at high Ca(2+) load, thereby reducing the driving force for Ca(2+) and Mg(2+) uptake. Cortical TAL showed a reduction in paracellular Ca(2+) and Mg(2+) permeabilities from 8.2 ± 0.7 to 6.2 ± 0.5 ∙ 10(-4) cm/s and from 4.8 ± 0.5 to 3.0 ± 0.2 · 10(-4) cm/s at control and high-Ca(2+) diet, respectively. Expression, localisation and regulation of claudins 10, 14, 16 and 19 differed along the corticomedullary axis: Towards the cortex, the main site of divalent cation reabsorption in TAL, high-Ca(2+) intake led to a strong upregulation of claudin-14 within TAL TJs while claudin-16 and -19 were unaltered. Towards the inner medulla, only claudin-10 was present in TAL TJ strands. In summary, high-Ca(2+) diet induced a reduction of divalent cation reabsorption via a diminution of NaCl transport and driving force in mTAL and via decreased paracellular permeabilities in cTAL. We reveal an important regulatory pattern along the corticomedullary axis and improve the understanding how the kidney disposes of detrimental excess Ca(2+).
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