ArticleOsteoarthritis and cartilage2016
Detecting new microRNAs in human osteoarthritic chondrocytes identifies miR-3085 as a human, chondrocyte-selective, microRNA.
Article in Osteoarthritis and cartilage, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Molecular mediators of motion: RNA-RBP networks in exercise-induced osteoarthritis protection.Frontiers in genetics · 2026Review
- Towards stratification in osteoarthritis: a review of the scientific terminology used in published basic research.BMC rheumatology · 2025Review
- Review
- Unveiling MiR-3085-3p as a modulator of cartilage degeneration in facet joint osteoarthritis: A novel therapeutic target.Journal of orthopaedic translation · 2025Article
- The Role of Genetics and Epigenetic Regulation in the Pathogenesis of Osteoarthritis.International journal of molecular sciences · 2023Review
- Consequences of genetic variants in miRNA genes.Computational and structural biotechnology journal · 2022Review
- miR-335-5P contributes to human osteoarthritis by targeting HBP1.Experimental and therapeutic medicine · 2021Article
- Regulation of microRNA-221, -222, -21 and -27 in articular cartilage subjected to abnormal compressive forces.The Journal of physiology · 2021Article
- The role of microRNA-3085 in chondrocyte function.Scientific reports · 2020Article
- microRNA-seq of cartilage reveals an overabundance of miR-140-3p which contains functional isomiRs.RNA (New York, N.Y.) · 2020Article
- The miRNA-mRNA interactome of murine induced pluripotent stem cell-derived chondrocytes in response to inflammatory cytokines.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Clusterin exerts a cytoprotective and antioxidant effect in human osteoarthritic cartilage.Aging · 2020Article
- The Transcription Factor-microRNA Regulatory Network during hESC-chondrogenesis.Scientific reports · 2020Article
- Article
- Long non-coding RNA XIST promotes extracellular matrix degradation by functioning as a competing endogenous RNA of miR-1277-5p in osteoarthritis.International journal of molecular medicine · 2019Article
- Epigenetics in osteoarthritis: Novel spotlight.Journal of cellular physiology · 2019Review
- miR-324-5p is up regulated in end-stage osteoarthritis and regulates Indian Hedgehog signalling by differing mechanisms in human and mouse.Matrix biology : journal of the International Society for Matrix Biology · 2019Article
- The Involvement of MicroRNAs in Osteoarthritis and Recent Developments: A Narrative Review.Mediterranean journal of rheumatology · 2018Review
- Deep sequencing and analyses of miRNAs, isomiRs and miRNA induced silencing complex (miRISC)-associated miRNome in primary human chondrocytes.Scientific reports · 2017Article
- Systems approaches in osteoarthritis: Identifying routes to novel diagnostic and therapeutic strategies.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
objectiveTo use deep sequencing to identify novel microRNAs (miRNAs) in human osteoarthritic cartilage which have a functional role in chondrocyte phenotype or function.
designA small RNA library was prepared from human osteoarthritic primary chondrocytes using in-house adaptors and analysed by Illumina sequencing. Novel candidate miRNAs were validated by northern blot and qRT-PCR. Expression was measured in cartilage models. Targets of novel candidates were identified by microarray and computational analysis, validated using 3'-UTR-luciferase reporter plasmids. Protein levels were assessed by western blot and functional analysis by cell adhesion.
resultsWe identified 990 known miRNAs and 1621 potential novel miRNAs in human osteoarthritic chondrocytes, 60 of the latter were expressed in all samples assayed. MicroRNA-140-3p was the most highly expressed microRNA in osteoarthritic cartilage. Sixteen novel candidate miRNAs were analysed further, of which six remained after northern blot analysis. Three novel miRNAs were regulated across models of chondrogenesis, chondrocyte differentiation or cartilage injury. One sequence (novel #11), annotated in rodents as microRNA-3085-3p, was preferentially expressed in cartilage, dependent on chondrocyte differentiation and, in man, is located in an intron of the cartilage-expressed gene CRTAC-1. This microRNA was shown to target the ITGA5 gene directly (which encodes integrin alpha5) and inhibited adhesion to fibronectin (dependent on alpha5beta1 integrin).
conclusionDeep sequencing has uncovered many potential microRNA candidates expressed in human cartilage. At least three of these show potential functional interest in cartilage homeostasis and osteoarthritis (OA). Particularly, novel #11 (microRNA-3085-3p) which has been identified for the first time in man.
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Registered trials
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