ArticleApplied and environmental microbiology2016
A Novel Bacteriophage Targeting Cronobacter sakazakii Is a Potential Biocontrol Agent in Foods.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- The biology of jumbo phages.Nature communications · 2026Review
- Protecting Newborns from Multidrug-Resistant Infections: The Emerging Role of Bacteriophages.Viruses · 2026Review
- Evaluation of the Novel Bacteriophage Chage1 and Its Endolysin LysCG1 as Biocontrol Agents againstJournal of microbiology and biotechnology · 2026Article
- Amino acid residues in the tail fiber differentiate the host specificity ofJournal of virology · 2025Article
- Targeted dual-receptor phage cocktail againstFrontiers in microbiology · 2024Article
- Characteristics on host specificity, infection, and temperature stability ofFood science and biotechnology · 2021Article
- Phage phiKZ-The First of Giants.Viruses · 2021Review
- Characterization and Genomic Analysis of PALS2, a NovelFrontiers in microbiology · 2021Article
- The morphological and biological characteristics of a virulent PI phage isolated from slaughterhouse sewage in Shiraz, Iran.Iranian journal of microbiology · 2020Article
- Multisubunit RNA Polymerases of Jumbo Bacteriophages.Viruses · 2020Review
- Controlling foodborne pathogens with natural antimicrobials by biological control and antivirulence strategies.Heliyon · 2020Review
- Identification and in vitro Characterization of a Novel Phage Endolysin that Targets Gram-Negative Bacteria.Microorganisms · 2020Article
- The NovelFrontiers in microbiology · 2019Article
- A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated AgainstFrontiers in microbiology · 2019Article
- Genomic Characterization of JumboFrontiers in microbiology · 2019Article
- To Be or Not To Be T4: Evidence of a Complex Evolutionary Pathway of Head Structure and Assembly in GiantFrontiers in microbiology · 2017Article
- Identification of Essential Genes in the Salmonella Phage SPN3US Reveals Novel Insights into Giant Phage Head Structure and Assembly.Journal of virology · 2016Article
- The genome of AR9, a giant transducing Bacillus phage encoding two multisubunit RNA polymerases.Virology · 2016Article
- Biocontrol and Rapid Detection of Food-Borne Pathogens Using Bacteriophages and Endolysins.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cronobacter sakazakii is an important pathogen that causes high mortality in infants. Due to its occasional antibiotic resistance, a bacteriophage approach might be an alternative effective method for the control of this pathogen. To develop a novel biocontrol agent using bacteriophages, the C. sakazakii-infecting phage CR5 was newly isolated and characterized. Interestingly, this phage exhibited efficient and relatively durable host lysis activity. In addition, a specific gene knockout study and subsequent complementation experiment revealed that this phage infected the host strain using the bacterial flagella. The complete genome sequence analysis of phage CR5 showed that its genome contains 223,989 bp of DNA, including 231 predicted open reading frames (ORFs), and it has a G+C content of 50.06%. The annotated ORFs were classified into six functional groups (structure, packaging, host lysis, DNA manipulation, transcription, and additional functions); no gene was found to be related to virulence or toxin or lysogen formation, but >80% of the predicted ORFs are unknown. In addition, a phage proteomic analysis using SDS-PAGE and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) revealed that seven phage structural proteins are indeed present, supporting the ORF predictions. To verify the potential of this phage as a biocontrol agent against C. sakazakii, it was added to infant formula milk contaminated with a C. sakazakii clinical isolate or food isolate, revealing complete growth inhibition of the isolates by the addition of phage CR5 when the multiplicity of infection (MOI) was 10(5).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.