ArticleBMC infectious diseases2015
The molecular characterisation of Escherichia coli K1 isolated from neonatal nasogastric feeding tubes.
Article in BMC infectious diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Global Extraintestinal Pathogenic Escherichia coli (ExPEC) Lineages.Clinical microbiology reviews · 2019Pooled it
- Antibiotics modulate Escherichia coli-derived bacterial extracellular vesicle production and their upregulation of ICAM-1 in human endothelial cells.Biology open · 2026Article
- Vimentin-NF-κB signaling contributing to IbeA-mediated adhesion, invasion, and biofilm formation duringFrontiers in immunology · 2026Article
- Research Progress on perinatalFrontiers in microbiology · 2026Review
- Antimicrobial susceptibility pattern of extended-spectrum β-lactamase producing gram-negative bacteria isolated from clinical samples: A cross-sectional hospital-based study.Journal of family medicine and primary care · 2025Article
- Diversity of Environmental Escherichia coli in Subtropical Freshwater Systems of South Africa.Current microbiology · 2025Article
- Escherichia Coli K1-colibactin meningitis induces microglial NLRP3/IL-18 exacerbating H3K4me3-synucleinopathy in human inflammatory gut-brain axis.Communications biology · 2025Article
- Molecular characterization of avian pathogenicVeterinary world · 2024Article
- Antimicrobial Effect of Diphenyl Ditelluride (PhTe)Indian journal of microbiology · 2024Article
- Therapeutic and Diagnostic Potential of a Novel K1 Capsule Dependent Phage, JSSK01, and Its Depolymerase in Multidrug-ResistantInternational journal of molecular sciences · 2024Article
- High prevalence of ESBL-producing E. coli phylogroup B2 clinical isolates in northeastern Thailand.BMC microbiology · 2024Article
- Preclinical validation of anMicrobiology spectrum · 2024Article
- Analysis of Retrospective Laboratory Data on the Burden of Bacterial Pathogens Isolated at the National Veterinary Research Institute Nigeria, 2018-2021.Veterinary sciences · 2023Article
- Phage Targeting Neonatal MeningitisInternational journal of molecular sciences · 2023Article
- A bioorthogonal chemistry approach to detect the K1 polysialic acid capsule inRSC chemical biology · 2023Article
- Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry AssociatedAntibiotics (Basel, Switzerland) · 2022Article
- A comparison of bacterial colonization between nasogastric and orogastric enteral feeding tubes in infants in the neonatal intensive care unit.Journal of perinatology : official journal of the California Perinatal Association · 2022Article
- Isolation and Characterization of Lytic Bacteriophages Active against Clinical Strains ofViruses · 2022Article
- Neonatal Feeding Tube Colonization and the Potential Effect on Infant Health: A Review.Frontiers in nutrition · 2022Review
- Close genetic linkage between human and companion animal extraintestinal pathogenicCurrent research in microbial sciences · 2022Article
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9 authors.
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Abstract
backgroundThe most common cause of Gram-negative bacterial neonatal meningitis is E. coli K1. It has a mortality rate of 10-15 %, and neurological sequelae in 30-50 % of cases. Infections can be attributable to nosocomial sources, however the pre-colonisation of enteral feeding tubes has not been considered as a specific risk factor.
methodsThirty E. coli strains, which had been isolated in an earlier study, from the residual lumen liquid and biofilms of neonatal nasogastric feeding tubes were genotyped using pulsed-field gel electrophoresis, and 7-loci multilocus sequence typing. Potential pathogenicity and biofilm associated traits were determined using specific PCR probes, genome analysis, and in vitro tissue culture assays.
resultsThe E. coli strains clustered into five pulsotypes, which were genotyped as sequence types (ST) 95, 73, 127, 394 and 2076 (Achman scheme). The extra-intestinal pathogenic E. coli (ExPEC) phylogenetic group B2 ST95 serotype O1:K1:NM strains had been isolated over a 2 week period from 11 neonates who were on different feeding regimes. The E. coli K1 ST95 strains encoded for various virulence traits associated with neonatal meningitis and extracellular matrix formation. These strains attached and invaded intestinal, and both human and rat brain cell lines, and persisted for 48 h in U937 macrophages. E. coli STs 73, 394 and 2076 also persisted in macrophages and invaded Caco-2 and human brain cells, but only ST394 invaded rat brain cells. E. coli ST127 was notable as it did not invade any cell lines.
conclusionsRoutes by which E. coli K1 can be disseminated within a neonatal intensive care unit are uncertain, however the colonisation of neonatal enteral feeding tubes may be one reservoir source which could constitute a serious health risk to neonates following ingestion.
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