ArticleG3 (Bethesda, Md.)2015
Examination of Endogenous Rotund Expression and Function in Developing Drosophila Olfactory System Using CRISPR-Cas9-Mediated Protein Tagging.
Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed.
- Tissue-specific transcriptomic analysis of accelerated reproductive development of immature Queensland fruit fly induced by raspberry ketone ingestion.BMC genomics · 2026Article
- Genomics of a sexually selected sperm ornament and female preference in Drosophila.Nature ecology & evolution · 2025Article
- The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons.G3 (Bethesda, Md.) · 2023Article
- Divergent expression of paralogous genes by modification of shared enhancer activity through a promoter-proximal silencer.Current biology : CB · 2022Article
- Evolution, developmental expression and function of odorant receptors in insects.The Journal of experimental biology · 2020Review
- Hedgehog signaling promotes lipolysis in adipose tissue through directly regulating Bmm/ATGL lipase.Developmental biology · 2020Article
- Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila.Journal of visualized experiments : JoVE · 2018Article
- Review
- Engineering the Drosophila Genome for Developmental Biology.Journal of developmental biology · 2017Review
- Article
- Application of CRISPR/Cas9 to the study of brain development and neuropsychiatric disease.Molecular and cellular neurosciences · 2017Review
- Transcriptional profiling of olfactory system development identifies distal antenna as a regulator of subset of neuronal fates.Scientific reports · 2017Article
- Editing the Neuronal Genome: a CRISPR View of Chromatin Regulation in Neuronal Development, Function, and Plasticity.The Yale journal of biology and medicine · 2016Review
- Ferritin Assembly in Enterocytes of Drosophila melanogaster.International journal of molecular sciences · 2016Article
- A Functionally Conserved Gene Regulatory Network Module Governing Olfactory Neuron Diversity.PLoS genetics · 2016Article
Corrections and comments
- Erratum issuedCorrigendum.2016
Authors and funding
4 authors.
Funding
Abstract
The zinc-finger protein Rotund (Rn) plays a critical role in controlling the development of the fly olfactory system. However, little is known about its molecular function in vivo. Here, we added protein tags to the rn locus using CRISPR-Cas9 technology in Drosophila to investigate its subcellular localization and the genes that it regulates . We previously used a reporter construct to show that rn is expressed in a subset of olfactory receptor neuron (ORN) precursors and it is required for the diversification of ORN fates. Here, we show that tagged endogenous Rn protein is functional based on the analysis of ORN phenotypes. Using this method, we also mapped the expression pattern of the endogenous isoform-specific tags in vivo with increased precision. Comparison of the Rn expression pattern from this study with previously published results using GAL4 reporters showed that Rn is mainly present in early steps in antennal disc patterning, but not in pupal stages when ORNs are born. Finally, using chromatin immunoprecipitation, we showed a direct binding of Rotund to a previously identified regulatory element upstream of the bric-a-brac gene locus in the developing antennal disc.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.