ArticleG3 (Bethesda, Md.)2015
Dynamics of Wolbachia pipientis Gene Expression Across the Drosophila melanogaster Life Cycle.
Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- A Meta-Analysis ofG3 (Bethesda, Md.) · 2020Pooled it
- Temperature-sensitive cytoplasmic incompatibility across divergent Wolbachia partly reflects cifB transcription, not endosymbiont density.Molecular biology and evolution · 2026Article
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- Temperature-sensitive cytoplasmic incompatibility across divergentbioRxiv : the preprint server for biology · 2026Article
- Counting cytoplasmic incompatibility factor mRNA using digital droplet PCR.Microbiology spectrum · 2026Article
- A co-speciation dilemma and a lifestyle transition with genomic consequences in Wolbachia of Neotropical Drosophila.BMC genomics · 2025Article
- The intracellular symbiont Wolbachia alters Drosophila development and metabolism to buffer against nutritional stress.PLoS genetics · 2025Article
- Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiontMicrobial genomics · 2025Article
- Evolutionary Diversification and Functions of the Candidate Male Killing Gene wmk.Genome biology and evolution · 2025Article
- Nucleotide composition shapes gene expression inmSystems · 2025Article
- Time-Course Gene Expression of 'Microorganisms · 2025Article
- OligoY pipeline for full Y chromosome painting.Briefings in bioinformatics · 2025Article
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- Improved RNA preparation for RNA-seq of the intracellular bacteriumFrontiers in microbiology · 2025Article
- Metatranscriptomics provide insights into the role of the symbiont Midichloria mitochondrii in Ixodes ticks.FEMS microbiology ecology · 2024Article
- Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes.Nature communications · 2024Article
- The cellular lives of Wolbachia.Nature reviews. Microbiology · 2023Review
- Wolbachia has subtle effects on thermal preference in highly inbred Drosophila melanogaster which vary with life stage and environmental conditions.Scientific reports · 2023Article
- SymbioticFrontiers in microbiology · 2023Review
- NewInternational journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
Symbiotic interactions between microbes and their multicellular hosts have manifold biological consequences. To better understand how bacteria maintain symbiotic associations with animal hosts, we analyzed genome-wide gene expression for the endosymbiotic α-proteobacteria Wolbachia pipientis across the entire life cycle of Drosophila melanogaster. We found that the majority of Wolbachia genes are expressed stably across the D. melanogaster life cycle, but that 7.8% of Wolbachia genes exhibit robust stage- or sex-specific expression differences when studied in the whole-organism context. Differentially-expressed Wolbachia genes are typically up-regulated after Drosophila embryogenesis and include many bacterial membrane, secretion system, and ankyrin repeat-containing proteins. Sex-biased genes are often organized as small operons of uncharacterized genes and are mainly up-regulated in adult Drosophila males in an age-dependent manner. We also systematically investigated expression levels of previously-reported candidate genes thought to be involved in host-microbe interaction, including those in the WO-A and WO-B prophages and in the Octomom region, which has been implicated in regulating bacterial titer and pathogenicity. Our work provides comprehensive insight into the developmental dynamics of gene expression for a widespread endosymbiont in its natural host context, and shows that public gene expression data harbor rich resources to probe the functional basis of the Wolbachia-Drosophila symbiosis and annotate the transcriptional outputs of the Wolbachia genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.