ArticleNucleic acids research2016
Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Meta-analysis Driven Strain Design for Mitigating Oxidative Stresses Important in Biomanufacturing.ACS synthetic biology · 2024Pooled it
- Optimizing recombinant mini proinsulin production via response surface method and microbioreactor screening.PloS one · 2025Article
- Unpredictability of the Fitness Effects of Antimicrobial Resistance Mutations Across Environments in Escherichia coli.Molecular biology and evolution · 2024Article
- Recent advances in genome-scale engineering inEngineering microbiology · 2024Review
- Article
- Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress.NPJ biofilms and microbiomes · 2021Article
- Recombineering and MAGE.Nature reviews. Methods primers · 2021Article
- Article
- Dominant resistance and negative epistasis can limit the co-selection of de novo resistance mutations and antibiotic resistance genes.Nature communications · 2020Article
- Stepwise evolution and convergent recombination underlie the global dissemination of carbapenemase-producing Escherichia coli.Genome medicine · 2020Article
- An sRNA Screen for Reversal of Quinolone Resistance inG3 (Bethesda, Md.) · 2020Article
- CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis.The Plant cell · 2019Article
- CRISPR/Cas9-Assisted Seamless Genome Editing in Lactobacillus plantarum and Its Application inApplied and environmental microbiology · 2019Article
- A versatile platform strain for high-fidelity multiplex genome editing.Nucleic acids research · 2019Article
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- A standardized workflow for surveying recombinases expands bacterial genome-editing capabilities.Microbial biotechnology · 2018Article
- Automated growth rate determination in high-throughput microbioreactor systems.BMC research notes · 2017Article
- CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability.Nucleic acids research · 2017Article
- Efficient engineering of chromosomal ribosome binding site libraries in mismatch repair proficient Escherichia coli.Scientific reports · 2017Article
- Rewriting the Genetic Code.Annual review of microbiology · 2017Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homologous recombination of single-stranded oligonucleotides is a highly efficient process for introducing precise mutations into the genome of E. coli and other organisms when mismatch repair (MMR) is disabled. This can result in the rapid accumulation of off-target mutations that can mask desired phenotypes, especially when selections need to be employed following the generation of combinatorial libraries. While the use of inducible mutator phenotypes or other MMR evasion tactics have proven useful, reported methods either require non-mobile genetic modifications or costly oligonucleotides that also result in reduced efficiencies of replacement. Therefore a new system was developed, Transient Mutator Multiplex Automated Genome Engineering (TM-MAGE), that solves problems encountered in other methods for oligonucleotide-mediated recombination. TM-MAGE enables nearly equivalent efficiencies of allelic replacement to the use of strains with fully disabled MMR and with an approximately 12- to 33-fold lower off-target mutation rate. Furthermore, growth temperatures are not restricted and a version of the plasmid can be readily removed by sucrose counterselection. TM-MAGE was used to combinatorially reconstruct mutations found in evolved salt-tolerant strains, enabling the identification of causative mutations and isolation of strains with up to 75% increases in growth rate and greatly reduced lag times in 0.6 M NaCl.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.