Evidence map›Paper›PMID 26496697›Full record

ArticleVirology2016

Biochemical analysis of the multifunctional vaccinia mRNA capping enzyme encoded by a temperature sensitive virus mutant.

Jessica Tate, Rachel L Boldt, Baron D McFadden, Susan M D'Costa, Nicholas M Lewandowski, Amber N Shatzer, Paul Gollnick, Richard C Condit

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jessica Tate *Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, P.O. Box 100266, Gainesville, FL 32610, United States.
Rachel L Boldt *Department of Biological Sciences, 609 Hochstetter Hall, State University of New York at Buffalo, Buffalo, NY 14260, United States.
Baron D McFaddenDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, P.O. Box 100266, Gainesville, FL 32610, United States.
Susan M D'CostaDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, P.O. Box 100266, Gainesville, FL 32610, United States.
Nicholas M LewandowskiDepartment of Biological Sciences, 609 Hochstetter Hall, State University of New York at Buffalo, Buffalo, NY 14260, United States.
Amber N ShatzerDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, P.O. Box 100266, Gainesville, FL 32610, United States.
Paul GollnickDepartment of Biological Sciences, 609 Hochstetter Hall, State University of New York at Buffalo, Buffalo, NY 14260, United States.
Richard C ConditDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, P.O. Box 100266, Gainesville, FL 32610, United States.
University of Florida · USUniversity at Buffalo, State University of New York · US

Funding

VACCINIA VIRUS BIOCHEMICAL GENETICSR01AI018094 · NIAID · UNIVERSITY OF FLORIDA · PI CONDIT, RICHARD C · 1985 to 2013
$4.6M
NIAID NIH HHS R01 AI018094
6 · The paper itself

Abstract

Prior biochemical analysis of the heterodimeric vaccinia virus mRNA capping enzyme suggests roles not only in mRNA capping but also in early viral gene transcription termination and intermediate viral gene transcription initiation. Prior phenotypic characterization of Dts36, a temperature sensitive virus mutant affecting the large subunit of the capping enzyme was consistent with the multifunctional roles of the capping enzyme in vivo. We report a biochemical analysis of the capping enzyme encoded by Dts36. Of the three enzymatic activities required for mRNA capping, the guanylyltransferase and methyltransferase activities are compromised while the triphosphatase activity and the D12 subunit interaction are unaffected. The mutant enzyme is also defective in stimulating early gene transcription termination and intermediate gene transcription initiation in vitro. These results confirm that the vaccinia virus mRNA capping enzyme functions not only in mRNA capping but also early gene transcription termination and intermediate gene transcription initiation in vivo.

Indexed as

AnimalsCell LineChlorocebus aethiopsHeLa CellsHumansMethyltransferasesmRNA GuanylyltransferasesMultienzyme ComplexesNucleoside-TriphosphataseNucleotidyltransferasesPhosphoric Monoester HydrolasesRNA, MessengerRNA, ViralTranscription Initiation, GeneticTranscription Termination, GeneticVaccinia viruscapping enzyme, vaccinia virusMethyltransferasesmRNA GuanylyltransferasesMultienzyme ComplexesNucleoside-TriphosphataseNucleotidyltransferasesPhosphoric Monoester HydrolasesRNA, MessengerRNA, ViralViral ProteinsCapping enzymemRNA cappingTranscription initiationTranscription terminationVaccinia virus

Identifiers

PMID26496697
PMCPMC4679637
OpenAlexW2206328114

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.