ArticlePloS one2015
Cooperative Effects of FOXL2 with the Members of TGF-β Superfamily on FSH Receptor mRNA Expression and Granulosa Cell Proliferation from Hen Prehierarchical Follicles.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- Micro RNA miR-726-3p targets CYB5A in Hen ovaries to modulate granulosa cell proliferation and differentiation.Scientific reports · 2025Article
- Hyperandrogenism in polycystic ovary syndrome augments Estrogen synthesis through AR-FOXL2-mediated activation of the aromatase gene in granulosa cells.Journal of ovarian research · 2025Article
- Mitochondrial Quality Control in Ovarian Function: From Mechanisms to Therapeutic Strategies.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Protective effect of luteinizing hormone on frozen-thawed ovarian follicles and granulosa cells.PloS one · 2025Article
- The regulatory roles of Smad2/3 protein andInternational journal of reproductive biomedicine · 2024Article
- Transcriptome comparative analysis of ovarian follicles reveals the key genes and signaling pathways implicated in hen egg production.BMC genomics · 2021Article
- Proteomic Analysis Identifies Potential Markers for Chicken Primary Follicle Development.Animals : an open access journal from MDPI · 2021Article
- Effects of RAC1 on Proliferation of Hen Ovarian Prehierarchical Follicle Granulosa Cells.Animals : an open access journal from MDPI · 2020Article
- Phytoestrogen genistein hinders ovarian oxidative damage and apoptotic cell death-induced by ionizing radiation: co-operative role of ER-β, TGF-β, and FOXL-2.Scientific reports · 2020Article
- Article
- Expression ofAnimals : an open access journal from MDPI · 2019Article
- Synergistic inhibition of csal1 and csal3 in granulosa cell proliferation and steroidogenesis of hen ovarian prehierarchical development†.Biology of reproduction · 2019Article
- The Role of microRNAs in Ovarian Granulosa Cells in Health and Disease.Frontiers in endocrinology · 2019Review
- Inhibitory effect of SLIT2 on granulosa cell proliferation mediated by the CDC42-PAKs-ERK1/2 MAPK pathway in the prehierarchical follicles of the chicken ovary.Scientific reports · 2018Article
- Review
- TGF-β signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis.Cell death & disease · 2016Article
- The Hippo/MST Pathway Member SAV1 Plays a Suppressive Role in Development of the Prehierarchical Follicles in Hen Ovary.PloS one · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Forkhead box L2 (FOXL2) is a member of the forkhead nuclear factor 3 gene family and plays an essential role in ovarian growth and maturation in mammals. However, its potential effects and regulative mechanism in development of chicken ovarian prehierarchical follicles remain unexplored. In this study, the cooperative effects of FOXL2 with activin A, growth differentiation factor-9 (GDF9) and follistatin, three members of the transforming growth factor beta (TGF-β) superfamily that were previously suggested to exert a critical role in follicle development was investigated. We demonstrated herein, using in-situ hybridization, Northern blot and immunohistochemical analyses of oocytes and granulosa cells in various sizes of prehierarchical follicles that both FOXL2 transcripts and FOXL2 proteins are predominantly expressed in a highly similar expression pattern to that of GDF9 gene. In addition, the FOXL2 transcript was found at lower levels in theca cells in the absence of GDF9. Furthermore, culture of granulosa cells (GCs) from the prehierarchical follicles (6-8 mm) in conditioned medium revealed that in the pcDNA3.0-FOXL2 transfected GCs, there was a more dramatic increase in FSHR mRNA expression after treatment with activin A (10 ng/ml) or GDF9 (100 ng/ml) for 24 h which caused a stimulatory effect on the GC proliferation. In contrast, a significant decrease of FSHR mRNA was detected after treatment with follistatin (50 ng/ml) and resulted in an inhibitory effect on the cell proliferation. The results of this suggested that FOXL2 plays a bidirectional modulating role involved in the intracellular FSHR transcription and GC proliferation via an autocrine regulatory mechanism in a positive or negative manner through cooperation with activin A and/or GDF9, and follistatin in the hen follicle development. This cooperative action may be mediated by the examined Smad signals and simultaneously implicated in modulation of the StAR, CCND2, and CYP11A1 expression.
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