ArticleJournal of natural products2015
Magnetic Ligand Fishing as a Targeting Tool for HPLC-HRMS-SPE-NMR: α-Glucosidase Inhibitory Ligands and Alkylresorcinol Glycosides from Eugenia catharinae.
Article in Journal of natural products, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 79 citations in OpenAlex.
- A Novel Platform Featuring Nanomagnetic Ligand Fishing Based on Fixed-Orientation Immobilized Magnetic Beads for Screening Potential Cyclooxygenase-2 Inhibitors fromMolecules (Basel, Switzerland) · 2024Article
- Biodiscoveries within the Australian plant genus Eremophila based on international and interdisciplinary collaboration: results and perspectives on outstanding ethical dilemmas.The Plant journal : for cell and molecular biology · 2022Review
- Chemical Characterization, α-Glucosidase, α-Amylase and Lipase Inhibitory Properties of the Australian Honey Bee Propolis.Foods (Basel, Switzerland) · 2022Article
- Drug discovery from natural products - Old problems and novel solutions for the treatment of neurodegenerative diseases.Journal of pharmaceutical and biomedical analysis · 2022Review
- An Integrated Strategy for the Detection, Dereplication, and Identification of DNA-Binding Biomolecules from Complex Natural Product Mixtures.Journal of natural products · 2021Article
- Magnetic ligand fishing using immobilized DPP-IV for identification of antidiabetic ligands in lingonberry extract.PloS one · 2021Article
- Recent advances in screening active components from natural products based on bioaffinity techniques.Acta pharmaceutica Sinica. B · 2020Review
- Bioassay-guided isolation and identification of gametocytocidal compounds from Artemisia afra (Asteraceae).Malaria journal · 2019Article
- Nanoenabled Bioseparations: Current Developments and Future Prospects.BioMed research international · 2019Review
- Fabrication of paper-based enzyme immobilized microarray by 3D-printing technique for screening α-glucosidase inhibitors in mulberry leaves and lotus leaves.Chinese medicine · 2019Article
- Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions.Frontiers in chemistry · 2019Review
- Magnetic beads-based neuraminidase enzyme microreactor as a drug discovery tool for screening inhibitors from compound libraries and fishing ligands from natural products.Journal of chromatography. A · 2018Article
- High-Resolution PTP1B Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of PTP1B Inhibitors fromMolecules (Basel, Switzerland) · 2018Article
- Article
- Ligand Fishing: A Remarkable Strategy for Discovering Bioactive Compounds from Complex Mixture of Natural Products.Molecules (Basel, Switzerland) · 2016Review
- Comparison of analytical techniques for the identification of bioactive compounds from natural products.Natural product reports · 2016Review
- Ligand fishing using new chitosan based functionalized Androgen Receptor magnetic particles.Journal of pharmaceutical and biomedical analysis · 2016Article
- Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays.Current pharmaceutical design · 2016Review
- Current status and contemporary approaches to the discovery of antitumor agents from higher plants.Biotechnology advancesReview
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
A bioanalytical platform combining magnetic ligand fishing for α-glucosidase inhibition profiling and HPLC-HRMS-SPE-NMR for structural identification of α-glucosidase inhibitory ligands, both directly from crude plant extracts, is presented. Magnetic beads with N-terminus-coupled α-glucosidase were synthesized and characterized for their inherent catalytic activity. Ligand fishing with the immobilized enzyme was optimized using an artificial test mixture consisting of caffeine, ferulic acid, and luteolin before proof-of-concept with the crude extract of Eugenia catharinae. The combination of ligand fishing and HPLC-HRMS-SPE-NMR identified myricetin 3-O-α-L-rhamnopyranoside, myricetin, quercetin, and kaempferol as α-glucosidase inhibitory ligands in E. catharinae. Furthermore, HPLC-HRMS-SPE-NMR analysis led to identification of six new alkylresorcinol glycosides, i.e., 5-(2-oxopentyl)resorcinol 4-O-β-D-glucopyranoside, 5-propylresorcinol 4-O-β-D-glucopyranoside, 5-pentylresorcinol 4-O-[α-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside, 5-pentylresorcinol 4-O-β-D-glucopyranoside, 4-hydroxy-3-O-methyl-5-pentylresorcinol 1-O-β-D-glucopyranoside, and 3-O-methyl-5-pentylresorcinol 1-O-[β-D-glucopyranosyl-(1→6)]-β-D-glucopyranoside.
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Registered trials
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