ArticlePloS one2015
Development of PCR-Based Markers to Determine the Sex of Kelps.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 61 citations in OpenAlex.
- Parallel loss of sexual reproduction in field populations of a brown alga sheds light on the mechanisms underlying the emergence of asexuality.Nature ecology & evolution · 2024Article
- A scaffolded and annotated reference genome of giant kelp (Macrocystis pyrifera).BMC genomics · 2023Article
- Selection drives convergent gene expression changes during transitions to co-sexuality in haploid sexual systems.Nature ecology & evolution · 2022Article
- Differential gene expression patterns during gametophyte development provide insights into sex differentiation in the dioicous kelp Saccharina japonica.BMC plant biology · 2021Article
- Seascape Genomics of the Sugar KelpGenes · 2020Article
- Review
- A key role for sex chromosomes in the regulation of parthenogenesis in the brown alga Ectocarpus.PLoS genetics · 2019Article
- Peptimapper: proteogenomics workflow for the expert annotation of eukaryotic genomes.BMC genomics · 2019Article
- Article
- Multiple gene movements into and out of haploid sex chromosomes.Genome biology · 2017Article
- High-density genetic map and identification of QTLs for responses to temperature and salinity stresses in the model brown alga Ectocarpus.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex discriminating genetic markers are commonly used to facilitate breeding programs in economically and ecologically important animal and plant species. However, despite their considerable economic and ecological value, the development of sex markers for kelp species has been very limited. In this study, we used the recently described sequence of the sex determining region (SDR) of the brown algal model Ectocarpus to develop novel DNA-based sex-markers for three commercially relevant kelps: Laminaria digitata, Undaria pinnatifida and Macrocystis pyrifera. Markers were designed within nine protein coding genes of Ectocarpus male and female (U/V) sex chromosomes and tested on gametophytes of the three kelp species. Seven primer pairs corresponding to three loci in the Ectocarpus SDR amplified sex-specific bands in the three kelp species, yielding at least one male and one female marker for each species. Our work has generated the first male sex-specific markers for L. digitata and U. pinnatifida, as well as the first sex markers developed for the genus Macrocystis. The markers and methodology presented here will not only facilitate seaweed breeding programs but also represent useful tools for population and demography studies and provide a means to investigate the evolution of sex determination across this largely understudied eukaryotic group.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.