ArticlePloS one2015
Accuracy of a New Platelet Count System (PLT-F) Depends on the Staining Property of Its Reagents.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
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- Automated cell count for blood component quality control.Transfusion · 2026Article
- Applications of Extended Platelet Profiles in Clinical Practice.Diseases (Basel, Switzerland) · 2026Review
- Evaluation of pre-dilution combined with optical/fluorescent platelet counting for correcting pseudothrombocytopenia.Frontiers in medicine · 2026Article
- Evaluation of Platelet Indices and Reticulated Platelets Using the ADVIA 2120 Analyzer in Patients with Acute Infection or Acute Coronary Syndrome, at Onset.Medical sciences (Basel, Switzerland) · 2025Article
- Overestimation of Automated Platelet Counts by Blast Fragments in Acute Hypercellular Leukemias: A Retrospective Study Comparing Impedance, Optical (PLT-O), Fluorescent (PLT-F), and CD41/CD61 Flow Cytometry Methods.International journal of laboratory hematology · 2025Article
- Cryoglobulinemia concurrent with Leishmania infantum infection in a dog and its interference with two automated hematology analyzers.Veterinary clinical pathology · 2025Article
- Hematological reference intervals for Danish crossbred Landrace Yorkshire Duroc (LYD) pigs used in biomedical research.Acta veterinaria Scandinavica · 2025Article
- Performance evaluation of the new Sysmex XR-Series haematology analyser.Practical laboratory medicine · 2024Article
- Reference guide for the diagnosis of adult primary immune thrombocytopenia, 2023 edition.International journal of hematology · 2024Article
- The immature platelet fraction affects the efficacy of platelet rich plasma therapy for knee osteoarthritis.Regenerative therapy · 2021Article
- Reticulated Platelets-Which Functions Have Been Established by In Vivo and In Vitro Data?Cells · 2021Review
- Establishment of improved review criteria for hematology analyzers in cancer hospitals.Journal of clinical laboratory analysis · 2021Article
- The Sysmex XN-L (XN-350) hematology analyzer offers a compact solution for laboratories in niche diagnostics.International journal of laboratory hematology · 2021Article
- Use of Middleware Data to Dissect and Optimize Hematology Autoverification.Journal of pathology informatics · 2021Article
- Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers.Journal of clinical medicine · 2020Article
- Platelet Counting: Ugly Traps and Good Advice. Proposals from the French-Speaking Cellular Hematology Group (GFHC).Journal of clinical medicine · 2020Review
- Immature Platelet Dynamics in Immune-Mediated Thrombocytopenic States.Frontiers in medicine · 2020Review
- Changes in novel haematological parameters following thermal injury: A prospective observational cohort study.Scientific reports · 2017Observational
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlatelet count is essential for the diagnosis and management of hemostasis abnormalities. Although existing platelet count methods installed in common hematology analyzers can correctly count platelets in normal blood samples, they tend to miscount platelets in some abnormal samples. The newly developed PLT-F channel in the XN-Series hematology analyzer (Sysmex) has been reported to be a reliable platelet count system, even in abnormal samples. However, how the PLT-F platelet counting system achieves such accuracy has not been described in scientific articles.
methodsIsolated platelets, erythrocytes, and fragmented erythrocytes were examined using an automated hematology analyzer. The samples were labeled by combining PLT-F reagents and anti-CD62p, CD63, Grp75, Calreticulin, CD41, or CD61 antibody, and analyzed using confocal laser scanning microscopy or flow cytometry.
resultsThe PLT-F system correctly discriminated platelets in erythrocytes. Its reagents strongly stained some intraplatelet organelles labeled with anti-Grp75, but only faintly stained the plasma membrane of both platelets and erythrocytes. Microscopic observation and flow cytometric examination revealed that all of these strongly stained cells were also labeled with platelet-specific anti-CD41 and anti-CD61 antibodies.
conclusionsThis study revealed that the staining property of the PLT-F reagents, by which platelets and fragmented erythrocytes are clearly distinguished, contributes to the platelet-counting accuracy of the PLT-F system.
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