ArticleBiosensors & bioelectronics2016
Sensitive detection of Escherichia coli O157:H7 using Pt-Au bimetal nanoparticles with peroxidase-like amplification.
Article in Biosensors & bioelectronics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed.
- Resolving the issue of non-specific substrate oxidation in nanozyme-assisted colorimetric assays.Analytical and bioanalytical chemistry · 2026Article
- Engineering Catalytic Nanozymes for Antimicrobial Food Systems: Structure-Activity Relationships, Safe-by-Design Principles, and Industrial Translation.Nanomaterials (Basel, Switzerland) · 2026Review
- Sensitive and quantitative biosensing technique based on NV centres-doped nanodiamonds applied to lateral flow assays.Scientific reports · 2026Article
- Portable Dual-Mode Biosensor for Quantitative Determination ofBiosensors · 2026Article
- New Frontiers for the Early Diagnosis of Cancer: Screening miRNAs Through the Lateral Flow Assay Method.Biosensors · 2025Review
- Platinum Nanozyme Probes for Cellular Imaging by Electron Microscopy.Small science · 2024Article
- A lateral flow assay for miRNA-21 based on CRISPR/Cas13a and MnOAnalytical and bioanalytical chemistry · 2024Article
- Triazine-based covalent-organic framework embedded with cuprous oxide as the bioplatform for photoelectrochemical aptasensing Escherichia coli.Mikrochimica acta · 2023Article
- Novel Vertical Flow Immunoassay with Au@PtNPs for Rapid, Ultrasensitive, and On-Site Diagnosis of Human Brucellosis.ACS omega · 2023Article
- Mulberry-like porous-hollow AuPtAg nanorods for electrochemical immunosensing of biomarker myoglobin.Mikrochimica acta · 2023Article
- Pd@Pt Nanodendrites as Peroxidase Nanomimics for Enhanced Colorimetric ELISA of Cytokines with Femtomolar Sensitivity.Chemosensors (Basel, Switzerland) · 2022Article
- Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications.Biosensors · 2022Review
- Nanozyme-Based Lateral Flow Immunoassay (LFIA) for Extracellular Vesicle Detection.Biosensors · 2022Article
- Exploration of nanozymes in viral diagnosis and therapy.Exploration (Beijing, China) · 2022Review
- Colorimetric Detection of Organophosphate Pesticides Based on Acetylcholinesterase and Cysteamine Capped Gold Nanoparticles as Nanozyme.Sensors (Basel, Switzerland) · 2021Article
- Bio-Specific Au/FeBiosensors · 2021Article
- Review
- A Review on Metal- and Metal Oxide-Based Nanozymes: Properties, Mechanisms, and Applications.Nano-micro letters · 2021Review
- Recent Advancements in Enzyme-Based Lateral Flow Immunoassays.Sensors (Basel, Switzerland) · 2021Review
- Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection.Trends in analytical chemistry : TRAC · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Escherichia coli O157:H7 is one of the most notorious foodborne pathogens causing serious disease at low infectious dose. To protect consumers from deadly foodborne E. coli O157:H7 infection, it is vital to develop a simple, reliable, sensitive and rapid method which can detect low level E. coli O157:H7 in foods at real-time. We have successfully developed a novel immunochromatographic assay (ICA) with enhanced sensitivity for the visual and quantitative detection of E. coli O157:H7. Sandwich-type immunoreactions were performed on the ICA, and Pt-Au bimetal nanoparticles (NPs) were accumulated on the test zone. The signal amplification is based on Pt-Au bimetal NPs possessing high peroxidase activity toward 3,3',5,5'-tetramethylbenzidine, which can produce characteristic colored bands and thus, enable visual detection of E. coli O157:H7 without instrumentation. The innovative aspect of this approach lies in the visualization and quantification of target pathogen through the detection of color intensity. Due to the excellent peroxidase activity of Pt-Au NPs, they emit strong visible color intensity in less than 1 min for visual observation even in low concentration range of E. coli O157:H7. Quantification was performed using a commercial assay meter. The sensitivity was improved more than 1000-folds compared to the conventional test strip based on colored gold-colloids. Although the feasibility was demonstrated using E. coli O157:H7 as a model analyte, this approach could be easily developed to be a universal signal amplification technique and applied to detection of a wide variety of foodborne pathogens and protein biomarkers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.