Evidence map›Paper›PMID 26496197›Full record

ArticlePloS one2015

Mantle Branch-Specific RNA Sequences of Moon Scallop Amusium pleuronectes to Identify Shell Color-Associated Genes.

Rong-Lian Huang, Zhe Zheng, Qing-Heng Wang, Xiao-Xia Zhao, Yue-Wen Deng, Yu Jiao, Xiao-Dong Du

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Rong-Lian HuangFishery College, Guangdong Ocean University, Zhanjiang, China; Laboratory of Marine Pearl Culture, Zhanjiang, China.
Zhe ZhengFishery College, Guangdong Ocean University, Zhanjiang, China; Laboratory of Marine Pearl Culture, Zhanjiang, China.
Qing-Heng WangFishery College, Guangdong Ocean University, Zhanjiang, China; Laboratory of Marine Pearl Culture, Zhanjiang, China.
Xiao-Xia ZhaoEnvironment Protection Monitoring Station, Environmental Protection Agency of Zhanjiang, Zhanjiang, China.
Yue-Wen DengFishery College, Guangdong Ocean University, Zhanjiang, China; Laboratory of Marine Pearl Culture, Zhanjiang, China.
Yu JiaoFishery College, Guangdong Ocean University, Zhanjiang, China; Laboratory of Marine Pearl Culture, Zhanjiang, China.
Xiao-Dong DuFishery College, Guangdong Ocean University, Zhanjiang, China; Laboratory of Marine Pearl Culture, Zhanjiang, China.
Zhanjiang Experimental Station · CNGuangdong Ocean University · CNShenyang Environmental Protection Bureau · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Amusium pleuronectes (Linnaeus) that secretes red- and white-colored valves in two branches of mantle tissues is an excellent model for shell color research. High-throughput transcriptome sequencing and profiling were applied in this project to reveal the detailed molecular mechanism of this phenotype differentiation. In this study, 50,796,780 and 54,361,178 clean reads were generated from the left branch (secreting red valve, RS) and right branch (secreting white valve, WS) using the Illumina Hiseq 2000 platform. De novo assembly generated 149,375 and 176,652 unigenes with an average length of 764 bp and 698 bp in RS and WS, respectively. Kyoto encyclopedia of genes and genomes (KEGG) metabolic pathway analysis indicated that the differentially expressed genes were involved in 228 signaling pathways, and 43 genes were significantly enriched (P<0.01). Nineteen of 20 differentially expressed vitellogenin genes showed significantly high expression in RS, which suggested that they probably played a crucial role in organic pigment assembly and transportation of the shell. Moreover, 687 crystal formation-related (or biomineralization-related) genes were detected in A. pleuronectes, among which 144 genes exhibited significant difference between the two branches. Those genes could be classified into shell matrix framework participants, crystal nucleation and growth-related elements, upstream regulation factors, Ca level regulators, and other classifications. We also identified putative SNP and SSR markers from these samples which provided the markers for genetic diversity analysis, genetic linkage, QTL analysis. These results provide insight into the complexity of shell color differentiation in A. pleuronectes so as valuable resources for further research.

Indexed as

AnimalsAnimal ShellsGene Expression ProfilingGene OntologyMolecular Sequence AnnotationPectinidaePigmentationRNA, MessengerSequence Analysis, RNATranscriptomeRNA, Messenger

Identifiers

PMID26496197
PMCPMC4619886
OpenAlexW2140726227

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.