Evidence map›Paper›PMID 26495844›Full record

ArticlePloS one2015

Membrane Cholesterol Modulates LOX-1 Shedding in Endothelial Cells.

Magda Gioia, Giulia Vindigni, Barbara Testa, Sofia Raniolo, Giovanni Francesco Fasciglione, Massimiliano Coletta, Silvia Biocca

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Magda GioiaDepartment of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata, Via Montpellier 1, 00133, Rome, Italy.
Giulia VindigniDepartment of Systems Medicine, University of Rome "Tor Vergata", Via Montpellier 1, 00133, Rome, Italy.
Barbara TestaDepartment of Systems Medicine, University of Rome "Tor Vergata", Via Montpellier 1, 00133, Rome, Italy.
Sofia RanioloDepartment of Systems Medicine, University of Rome "Tor Vergata", Via Montpellier 1, 00133, Rome, Italy.
Giovanni Francesco FasciglioneDepartment of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata, Via Montpellier 1, 00133, Rome, Italy.
Massimiliano ColettaDepartment of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata, Via Montpellier 1, 00133, Rome, Italy.
Silvia BioccaDepartment of Systems Medicine, University of Rome "Tor Vergata", Via Montpellier 1, 00133, Rome, Italy.
University of Rome Tor Vergata · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a scavenger receptor responsible for ox-LDL recognition, binding and internalization, which is up-regulated during atherogenesis. Its activation triggers endothelium dysfunction and induces inflammation. A soluble form of LOX-1 has been identified in the human blood and its presence considered a biomarker of cardiovascular diseases. We recently showed that cholesterol-lowering drugs inhibit ox-LDL binding and internalization, rescuing the ox-LDL induced apoptotic phenotype in primary endothelial cells. Here we have investigated the molecular bases of human LOX-1 shedding by metalloproteinases and the role of cell membrane cholesterol on the regulation of this event by modulating its level with MβCD and statins. We report that membrane cholesterol affects the release of different forms of LOX-1 in cells transiently and stably expressing human LOX-1 and in a human endothelial cell line (EA.hy926). In particular, our data show that i) cholesterol depletion triggers the release of LOX-1 in exosomes as a full-length transmembrane isoform and as a truncated ectodomain soluble fragment (sLOX-1); ii) endothelial cells secrete a soluble metalloproteinase which induces LOX-1 ectodomain shedding and iii) long term statins treatment enhances sLOX-1 proteolytic shedding.

Indexed as

AnimalsCell MembraneChlorocebus aethiopsCHO CellsCholesterolCOS CellsCricetinaeCricetulusEndothelial CellsExosomesHEK293 CellsHumansLipoproteins, LDLMatrix Metalloproteinase 1Matrix Metalloproteinase 2Matrix Metalloproteinase 9CholesterolLipoproteins, LDLMatrix Metalloproteinase 1Matrix Metalloproteinase 2Matrix Metalloproteinase 9MMP1 protein, humanMMP2 protein, humanMMP9 protein, humanOLR1 protein, humanoxidized low density lipoproteinScavenger Receptors, Class ETIMP1 protein, humanTissue Inhibitor of Metalloproteinase-1

Identifiers

PMID26495844
PMCPMC4619672
OpenAlexW2157776118

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.